Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular.

Slides:

Advertisements

Similar presentations

Vascular endothelial growth factor A and its two receptors in human preantral follicles from fetuses, girls, and women Ronit Abir, Ph.D., Asangla Ao,

Advertisements

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Mira Park, Ph. D. , Dae-Shik Suh, M. D. , Kangseok Lee, Ph. D

Effects of testosterone, dihydrotestosterone, and 17β-estradiol on human ovarian tissue survival in culture Marjut Otala, M.Sc., Sirpa Mäkinen, M.Sc.,

Jong Yeob Choi, Ph. D. , Min Wha Jo, M. S. , Eun Young Lee, M. S

Developmental programming: prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles Almudena Veiga-Lopez,

Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the.

Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells Christine Chabrolle, M.D., Lucie.

Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition Monica Faut, Evelin Mariel Elia,

Cigarette smoke condensate exposure delays follicular development and function in a stage-dependent manner Jean Clair Sadeu, M.D., Ph.D., Warren G. Foster,

2-Methoxyestradiol causes functional repression of transforming growth factor β3 signaling by ameliorating Smad and non-Smad signaling pathways in immortalized.

Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway Tatsuo Nakahara, M.D., Akira Iwase, M.D., Ph.D.,

Israel Ortega, M. D. , Anna Sokalska, M. D. , Ph. D. , Jesus A

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells

MRNA expression pattern of insulin-like growth factor components of granulosa cells and cumulus cells in women with and without polycystic ovary syndrome.

Concentrations of stromal cell-derived factor-1 and vascular endothelial growth factor in relation to the diameter of human follicles Akemi Nishigaki,

Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle Rebecca S. Brogan, Ph.D., Scott Mix, Muraly.

Curcumin, a nutritional supplement with antineoplastic activity, enhances leiomyoma cell apoptosis and decreases fibronectin expression Minnie Malik,

Dienogest enhances autophagy induction in endometriotic cells by impairing activation of AKT, ERK1/2, and mTOR JongYeob Choi, Ph.D., MinWha Jo, M.S.,

Hadas Bar-Joseph, Ph. D. , Ido Ben-Ami, M. D. , Ph. D

Direct effect of macrophage migration inhibitory factor on sperm function: possible involvement in endometriosis-associated infertility Cédric Carli,

Expression and localization of transcription factor Ets-1 in the rat ovary during the estrous cycle and pregnancy Xiaohui Xiao, M.S., Aiqin Liu, Ph.D.,

Antonino Forabosco, M.D., Ph.D., Chiarella Sforza, M.D., Ph.D.

Guoying Dong, B. S. , Yueshuai Guo, B. S. , Huarong Cao, B. S

The antifibrotic drug halofuginone inhibits proliferation and collagen production by human leiomyoma and myometrial smooth muscle cells Meagan M. Grudzien,

Recombinant H2 relaxin inhibits apoptosis and induces cell proliferation in cultured leiomyoma cells without affecting those in cultured normal myometrial.

Dopamine receptor 2 activation inhibits ovarian vascular endothelial growth factor secretion in an ovarian hyperstimulation syndrome (OHSS) animal model:

Derek K. Lobb, Ph.D. Fertility and Sterility

Expression of growth differentiation factor-9 and bone morphogenetic protein-15 in oocytes and cumulus granulosa cells of patients with polycystic ovary.

Western-style diet, with and without chronic androgen treatment, alters the number, structure, and function of small antral follicles in ovaries of young.

Growth differentiation factor 3 is induced by bone morphogenetic protein 6 (BMP-6) and BMP-7 and increases luteinizing hormone receptor messenger RNA.

Vascular density and distribution of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) are significantly higher in patients with.

Estradiol protects clomiphene citrate–induced apoptosis in ovarian follicular cells and ovulated cumulus–oocyte complexes Shail K. Chaube, Ph.D., Pramod.

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Kisspeptin stimulates progesterone secretion via the Erk1/2 mitogen-activated protein kinase signaling pathway in rat luteal cells Jing Peng, M.D., Min.

A new step toward the artificial ovary: survival and proliferation of isolated murine follicles after autologous transplantation in a fibrin scaffold

Dietary glycine does not affect physiological angiogenesis and reproductive function, but inhibits apoptosis in endometrial and ovarian tissue by down-regulation.

Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility Sara Correia, M.S.,

Pathophysiologic features of “thin” endometrium

Volume 68, Issue 4, Pages (October 2005)

Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine model Anne-Sophie Van Eyck, M.D., Caroline.

Monocyte chemotactic protein-1 (MCP-1), its receptor, and macrophages in the perifollicular stroma during the human ovulatory process Pernilla Dahm-Kähler,

Carla C. Maganhin, Ph. D. , Ricardo S. Simões, Ph. D. , Luiz F. P

Padmasana Singh, Ph. D. , Amitabh Krishna, Ph. D

Patricia Miyuki Tsuribe, Ph. D. , Carlos Alberto Monte Gobbo, M. D

Progress toward “in vivo virtual histology” of ovarian follicles and corpora lutea by ultrasound biomicroscopy Pilar Pallares, M.V.D., Claudia Letelier,

Gene injections of vascular endothelial growth factor and growth differentiation factor-9 stimulate ovarian follicular development in immature female.

Temporal window in which exposure to estradiol permanently modifies ovarian function causing polycystic ovary morphology in rats Gonzalo Cruz, Ph.D.,

Doxycycline inhibits vascular leakage and prevents ovarian hyperstimulation syndrome in a murine model Ofer Fainaru, M.D., Ph.D., Mark D. Hornstein,

Granulocyte colony-stimulating factor in conjunction with vascular endothelial growth factor maintains primordial follicle numbers in transplanted mouse.

Is anti-Müllerian hormone a marker of acute cyclophosphamide-induced ovarian follicular destruction in mice pretreated with cetrorelix? Hyacinth N. Browne,

Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo.

Glial cell line–derived neurotrophic factor (GDNF) and its receptors in human ovaries from fetuses, girls, and women Jacob Farhi, M.D., Asangla Ao, Ph.D.,

Bone morphogenetic protein-6 stimulates gene expression of follicle-stimulating hormone receptor, inhibin/activin β subunits, and anti-Müllerian hormone.

Emre Seli, M. D. , Ozlem Guzeloglu-Kayisli, Ph. D. , Umit A

Müllerian-inhibiting substance inhibits cytochrome P450 aromatase activity in human granulosa lutein cell culture Michael P. Grossman, M.D., Steven T.

Fertility and Sterility

Volume 61, Issue 2, Pages (February 2002)

Romidepsin reduces histone deacetylase activity, induces acetylation of histones, inhibits proliferation, and activates apoptosis in immortalized epithelial.

Prostaglandin-endoperoxide synthase (PTGS1 and PTGS2) expression and prostaglandin production by normal monkey ovarian surface epithelium Rafael A. Cabrera,

Exogenous androstenedione induces formation of follicular cysts and premature luteinization of granulosa cells in the ovary Yuki Okutsu, M.D., Masanori.

Follicular blood flow is a better predictor of the outcome of in vitro fertilization–embryo transfer than follicular fluid vascular endothelial growth.

Ovarian expression of markers associated with proliferation or apoptosis in women with diminished ovarian reserve Víctor Vital-Reyes, M.D., M.Sc., Cristina.

JongYeob Choi, Ph. D. , MinWha Jo, M. S. , EunYoung Lee, M. S

Katharina Hancke, M. D. , Oliver Strauch, V. M. D

Hsien-An Pan, M. D. , Yue-Shan Lin, M. D. , Ko-Hung Lee, M. D

Sodium nitroprusside treatment during the superovulation process improves ovarian response and ovarian expression of vascular endothelial growth factor.

Direct injection of vascular endothelial growth factor into the ovary of mice promotes follicular development Ramiro Quintana, M.D., Laura Kopcow, M.D.,

Cem Fiçicioǧlu, M. D. , Tayfun Kutlu, M. D. , Elif Baglam, M. D

Ovarian follicular volume and follicular surface area are better indicators of follicular growth and maturation, respectively, than is follicular diameter

Presentation transcript:

Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway Dalhia Abramovich, Ph.D., Griselda Irusta, Ph.D., Fernanda Parborell, Ph.D., Marta Tesone, Ph.D. Fertility and Sterility Volume 93, Issue 5, Pages 1369-1377 (March 2010) DOI: 10.1016/j.fertnstert.2009.01.127 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Effect of Trap treatment on PCNA protein levels in follicular cells. Upper panel: Densitometric quantification of PCNA in control and Trap-treated ovaries 48 h after injection. After homogenization, proteins were extracted, subjected to 12% SDS-PAGE, and transferred onto nitrocellulose membranes. Bars represent mean ± SEM. Each protein was normalized to actin B (n = 8; ∗P<.05; paired student t-test). Lower panel: Representative immunoblots of PCNA content in antral follicles from control and Trap-treated ovaries. Fertility and Sterility 2010 93, 1369-1377DOI: (10.1016/j.fertnstert.2009.01.127) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Effect of Trap treatment on the percentage of follicular cells positively immunostained for PCNA. Upper panel: Representative fields of ovarian sections immunostained for PCNA (100×). (A) Control ovary and (B) Trap-treated ovary. A portion of each section shows the detail observed at higher magnification (400×). Lower panel: PCNA expression analysis. (A) granulosa cells and (B) theca cells. Proliferation index was determined by counting labeled cells at 400× in randomly selected fields of antral follicles. Data are expressed as mean ± SEM. Four sections per ovary were analyzed (six ovaries/group). ∗P<.05; paired student t-test; n = 5. EAF = early antral follicle; GC = granulosa cell; TC = theca cell. Fertility and Sterility 2010 93, 1369-1377DOI: (10.1016/j.fertnstert.2009.01.127) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Effect of Trap treatment on cleavage of caspase 3 protein in antral follicles. Upper panel: Densitometric quantification of the caspase 3 active fragment p17 follicular content. Caspase 3 protein was visualized by using an anti-caspase 3 antibody that recognizes the procaspase and the p20, p17 and p11 fragments. Data indicate mean ± SEM normalized to actin B. (n = 8; ∗P<.05; paired student t-test). Lower panel: Representative immunoblot of caspase 3 protein content in antral follicles from control and Trap-treated ovaries. Fertility and Sterility 2010 93, 1369-1377DOI: (10.1016/j.fertnstert.2009.01.127) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Effect of Trap treatment on phosphorylation of AKT and BAD proteins in antral follicles. (A, upper panel) Densitometric quantification of pAKT/AKT in control and Trap-treated ovaries 4 and 8 h after injection. Bars represent mean ± SEM. Each protein was normalized to actin B (n = 8; ∗P<.05; paired student t-test). (Lower panel) Representative immunoblots of AKT and pAKT content in antral follicles from control and Trap-treated ovaries. (B, upper panel) Densitometric quantification of pBAD/BAD in control and Trap-treated ovaries 4 and 8 h after injection. Bars represent mean ± SEM. Each protein was normalized to actin B (n = 5; ∗P<.05; Paired Student t-test). (Lower panel) Representative immunoblots of BAD and pBAD content in antral follicles from control and Trap-treated ovaries. Fertility and Sterility 2010 93, 1369-1377DOI: (10.1016/j.fertnstert.2009.01.127) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Von Willebrand factor immunostaining in ovaries from control and Trap-treated rats. (Upper panel) Vessel networks and blood vessels marked with Anti-von Willebrand factor antibody in (A) control and (B) Trap-treated ovaries. (Lower panel) The percentage of endothelial cell area was determined by counting labeled cells in randomly selected 100X fields of ovarian sections (five sections/ovary, eight ovaries/group). Data are expressed as mean ± SEM (magnification 100×). EAF = early antral follicle; AtF = atretic follicle. Fertility and Sterility 2010 93, 1369-1377DOI: (10.1016/j.fertnstert.2009.01.127) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions