Nucleotide excision repair polymorphisms, polycyclic aromatic hydrocarbon exposure, and their effects on sperm deoxyribonucleic acid damage and male factor.

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Nucleotide excision repair polymorphisms, polycyclic aromatic hydrocarbon exposure, and their effects on sperm deoxyribonucleic acid damage and male factor infertility Aihua Gu, M.D., Ph.D., Guixiang Ji, Ph.D., Pengfei Zhu, M.S., Yong Zhou, Ph.D., Guangbo Fu, M.D., Yankai Xia, Ph.D., Ling Song, M.S., Shoulin Wang, Ph.D., Xinru Wang, Ph.D. Fertility and Sterility Volume 94, Issue 7, Pages 2620-2625.e5 (December 2010) DOI: 10.1016/j.fertnstert.2010.04.059 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Sperm PAH-DNA adducts were detected by an immunofluorescence staining with phycoerythrin-labeled antibody (orange); sperm DNA was counterstained with 6-diamino-2-phenylindole (DAPI) (blue). (A, C, E) The sperm staining of an infertile subject. (B, D, E) The same sample treated with 10 μmol/L benzo-(α)-pyrene (BPDE), set up as the positive control. Fertility and Sterility 2010 94, 2620-2625.e5DOI: (10.1016/j.fertnstert.2010.04.059) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Detection of sperm DNA damage with use of TUNEL assay Detection of sperm DNA damage with use of TUNEL assay. Histogram data of M2 values represented the fluorescein isothiocyanate conjugate fluorescence-labeled DNA-damaged spermatozoa. Two samples with TUNEL values of (A) 5.4% and (B) 24.9% are illustrated. Fertility and Sterility 2010 94, 2620-2625.e5DOI: (10.1016/j.fertnstert.2010.04.059) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Damage in DNA in ejaculates from 620 patients Damage in DNA in ejaculates from 620 patients. (A) TUNEL value without logarithmic transformation. (B) TUNEL value with logarithmic transformation (ln TUNEL). Fertility and Sterility 2010 94, 2620-2625.e5DOI: (10.1016/j.fertnstert.2010.04.059) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Distribution of urine CR-adjusted 1-hydroxypyrene (1-OHP) levels in 620 patients. (A) 1-hydroxypyrene levels without logarithmic transformation. (B) 1-Hydroxypyrene levels with logarithmic transformation. Fertility and Sterility 2010 94, 2620-2625.e5DOI: (10.1016/j.fertnstert.2010.04.059) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Association between logarithm of the sperm DNA damage and logarithm of urinary 1-hydroxypyrene (1-OHP). Adjusting for age, smoking, drinking, tea consumption, vitamin use, and abstinence time, the urinary 1-hydroxypyrene levels were associated significantly with sperm DNA damage with use of a linear regression: β coefficients = 0.183, P<.001. Fertility and Sterility 2010 94, 2620-2625.e5DOI: (10.1016/j.fertnstert.2010.04.059) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions