Figure S2 Supplementary data a b CCA1 CCA2 CCA4 CCA3 CCA5 EGFR HER2 Figure S2. EGFR and c-Myc proteins are overexpressed to similar levels in F0 and corresponding F1 tumors. (a) IHC staining demonstrates relatively high levels of EGFR expression in CCA1, 3 and 4. And moderate levels of c-Myc expression in F0 and F1 in CCA1 and CCA2 tumors. Scale bar = 20µm. Total expression index values are shown below each image. (b) Normal pancreas tissue expresses moderate levels of EGFR, but no detectable levels of HER2 or c-Myc protein. Scale bar for 20x=20µm; for 40x=10µm. Expression indices of EGFR, HER2 and c-Myc calculated as described in Materials and Methods. a b CCA1 CCA2 CCA4 CCA3 CCA5 EGFR HER2 c-Myc Primary Tumor (F0) PDX (F1) 250 200 <50 <50 120 80 170 140 0 0 <50 <50 285 250 <50 <50 270 225 105 150 0 0 160 200 240 200 100 60 <50 <50 20x 40x 150