Howard P. Greisler, MD, Joan Ellinger, Scott C. Henderson, Anne M

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Presentation transcript:

The effects of an atherogenic diet on macrophage/biomaterial interactions Howard P. Greisler, MD, Joan Ellinger, Scott C. Henderson, Anne M. Shaheen, Wilson H. Burgess, PhD, Dae Un Kim, MD, Tina M. Lam, MD Journal of Vascular Surgery Volume 14, Issue 1, Pages 10-23 (July 1991) DOI: 10.1016/0741-5214(91)90149-O Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Flow chart of experimental design. Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 A, Phase-contrast photomicrograph of peritoneal macrophages harvested from normal New Zealand white rabbits after 6 weeks in culture with PG910. Intracytoplasmic PG910 inclusions can be seen. (Original magnification × 500.) B, Phase-contrast photomicrograph of peritoneal macrophages harvested from atherosclerotic New Zealand white rabbits cultured for 8 weeks in the presence of PG910. Intracytoplasmic PG910 inclusions can be seen. No gross morphologic differences are seen comparing the macrophages from the two groups of rabbits. (Original magnification × 500.) Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 A, Photomicrograph from thoracic aorta of an atherosclerotic New Zealand white rabbit showing greatly thickened intima containing abundant foam cells. The elastic laminae of the media can be seen in the lower portion; the media contains smooth muscle cells with vacuolated cytoplasm. (Hematoxylin-eosin stain; original magnification × 400.) B, Higher magnification of intima outlined within box in A. (Hematoxylin-eosin stain; original magnification × 400.) Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 BALB/c3T3 fibroblast bioassay results. Studies with conditioned media from macrophages harvested from normal New Zealand white rabbits are in the top two panels and those studies with conditioned media from macrophages harvested from atherosclerotic New Zealand white rabbits in the lower two panels. The panels on the left show the results of each group of conditioned media in each successive week, expressed as mean counts per minute sample ÷ mean counts per minute fetal bovine serum positive control × 100. The right sided panels show the normalized data in which each week's media from the groups with prosthetic material is normalized by dividing its mean counts per minute by that of the media from the macrophages grown in the absence of prosthetic material, thus normalizing to polystyrene control values. Values > 1 indicate additional stimulation of mitogen release by the presence of the biomaterial, whereas values < 1 indicate less mitogen release than is seen in the absence of that biomaterial. (mean ± SD) Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Atherosclerotic rabbit aortic smooth muscle cell bioassay results. Studies that used conditioned media from macrophages harvested from normal New Zealand white rabbits are in the top two panels, and those studies that used conditioned media from macrophages harvested from atherosclerotic New Zealand white rabbits in the lower two panels. The panels on the left show the results of each group of conditioned media in each successive week, expressed as mean counts per minute sample ÷ mean counts per minute fetal bovine serum positive control × 100. The right sided panels show the normalized data in which each week's media from the groups with prosthetic material is normalized by dividing its mean counts per minute by that of the media from the macrophages grown in the absence of prosthetic material, thus normalizing to polystyrene control values. Values > 1 indicate additional stimulation of mitogen release by the presence of the biomaterial while values < 1 indicate less mitogen release than is seen in the absence of that biomaterial. (mean ± SD) Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Results of the LE-II endothelial cell bioassay. The panels on the left show the results by use of media collected during weeks 5 to 10 after exposure of macrophages harvested from normal New Zealand white rabbits to either no graft material, PG910, or Dacron. The lower left panel shows these data for successive weeks expressed as percent increase above quiescent (PBS) levels and the panel in the upper left shows these data normalized to the no graft material (polystyrene alone) control groups. The panels to the right indicate the same data collected on media from macrophages harvested from atherosclerotic New Zealand white rabbits, these conditioned media groups studied during weeks 2 to 12. Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 7 Results of the LE-II endothelial cell bioassay in which media from macrophages harvested from atherosclerotic New Zealand white rabbits and collected in weeks 2 to 12 were added to quiescent LE-II cells. Stimulation of cell proliferation (control and PG910) is shown in relation to the fetal bovine serum positive control. The inhibition of thymidine uptake by the media from the group containing Dacron is shown in relation to the PBS negative control. Journal of Vascular Surgery 1991 14, 10-23DOI: (10.1016/0741-5214(91)90149-O) Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions