Mean and difference Raman spectra of spleen tissue from endotoxemic mice and sham mice and PCA-LDA. Mean and difference Raman spectra of spleen tissue from endotoxemic mice and sham mice and PCA-LDA. (A) Mean Raman spectra of spleen tissue of sham C57BL/6 mice and C57BL/6 mice 1 d post–LPS treatment, difference spectrum between control and LPS-treated mice spleen tissue (label on right y-axis), and loading of PCA-LDA model for spleen tissue section differentiation of control and LPS-treated mice (label on right y-axis). (B) PCA-LDA histogram plot. The biological experiments were repeated four times; in each repetition one mouse per condition was used (in total four mice per condition; both female and male mice were used) (see Supplemental Table IB). Raman spectroscopic investigation was performed on three different days. Three tissue slices were investigated per spleen. Analysis has been done on pooled samples. Anuradha Ramoji et al. ImmunoHorizons 2019;3:45-60 Copyright © 2019 The Authors