Variable levels of cell surface and intracellular marker expression by HuT78 and HuT102 cells. Variable levels of cell surface and intracellular marker.

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Variable levels of cell surface and intracellular marker expression by HuT78 and HuT102 cells. Variable levels of cell surface and intracellular marker expression by HuT78 and HuT102 cells. (A) Hut78 and (B) HuT102 cells were maintained in cRPMI until harvest followed by staining for flow cytometry assay using specific Abs for the indicated cell surface and intracellular markers. Dead cells were eliminated from analysis based on their loss of membrane integrity and, thus, inclusion of a dead cell dye. The data shown represent live HuT cells on dot plots and histograms. On histograms, the levels of specific marker’s expression are shown in black line with transparent background, whereas an individual for each marker isotype control Ab stain is shown in solid gray color. The flow cytometry analysis data of one out of three experiments are shown. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors