Brenton T. Tan, Roger A. Warnke, Daniel A. Arber

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Presentation transcript:

The Frequency of B- and T-Cell Gene Rearrangements and Epstein-Barr Virus in T-Cell Lymphomas Brenton T. Tan, Roger A. Warnke, Daniel A. Arber The Journal of Molecular Diagnostics Volume 8, Issue 4, Pages 466-475 (September 2006) DOI: 10.2353/jmoldx.2006.060016 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Histology of representative AILT and PTCL-NOS cases. All panels shown at identical magnification. A–F: AILT with EBV-associated large B-cell proliferation stained with H&E (A), immunohistochemistry with anti-CD5 (B), anti-CD20 (C), in situ hybridization for EBV (D), anti-CD21 (E), or anti-CD10 (F). G and H: PTCL-NOS stained with H&E (G) or examined by in situ hybridization for EBV (H). Images were captured with a Nikon Eclipse E1000 microscope (Nikon, Tokyo, Japan) using a ×20/0.75 objective lens (Nikon) and a Spot CCD camera (Diagnostic Instruments, McHenry, IL) with Spot software version 4.0.5 for image acquisition. The Journal of Molecular Diagnostics 2006 8, 466-475DOI: (10.2353/jmoldx.2006.060016) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Electropherogram of PCR for TCRγ rearrangements. The x axis indicates DNA length in nucleotides. The y axis indicates relative fluorescence units. Shown is electrophoresis of products from one multiplex tube, which contains primers to amplify Vγ1-8 and/or Vγ10 rearrangements, with expected product sizes above the x axis. The case was interpreted as positive with a clonal peak detected by Vγ10 primers (arrow) and polyclonal signals detected by Vγ1-8 primers. The Journal of Molecular Diagnostics 2006 8, 466-475DOI: (10.2353/jmoldx.2006.060016) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Electropherograms of PCRs for IGH rearrangements. The x axis indicates DNA length in nucleotides. The y axis indicates relative fluorescence units. Shown are electrophoresis of products from three multiplex tubes, which contain primers targeting the FR1 (A), FR2 (B), and FR3 regions (C), with expected product sizes above the x axis. The case was interpreted as positive with clonal peaks detected by FR1 and FR2 primers (arrows) and polyclonal signals detected by FR3 primers. The Journal of Molecular Diagnostics 2006 8, 466-475DOI: (10.2353/jmoldx.2006.060016) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions