Fernando Henrique Biase, Ph. D. , Lúcia Martelli, M. D. , Ph. D

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Messenger RNA expression of Pabpnl1 and Mbd3l2 genes in oocytes and cleavage embryos Fernando Henrique Biase, Ph.D., Lúcia Martelli, M.D., Ph.D., Renato Puga, M.Sc., Silvana Giuliatti, Ph.D., Weruska Karyna Freitas Santos-Biase, M.Sc., Giovana Krempel Fonseca Merighe, Ph.D., Flávio Vieira Meirelles, Ph.D. Fertility and Sterility Volume 93, Issue 8, Pages 2507-2512 (May 2010) DOI: 10.1016/j.fertnstert.2009.08.051 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Representative reverse-transcription polymerase chain reaction detection of poly(A)-binding protein nuclear-like 1 (Pabpnl1) and methyl-CpG–binding domain protein 3–like 2 (Mbd3l2) transcripts in bovine tissues and cells. Agarose gel containing ethidium bromide showing the amplified fragments for the (A) Pabpnl1, (B) Mbd3l2, and (C) glyceraldeyde 3-phosphate dehydrogenase genes. Lanes: 1) 50-bp DNA marker; 2) immature and in vitro–matured oocytes; 3) preimplantation embryos; 4) brain; 5) corpus luteum; 6) cumulus cells; 7) fibroblast; 8) heart; 9) kidney; 10) leukocyte; 11) liver; 12) lung; 13) muscle; 14) ovary; 15) spleen; 16) stomach; 17) testis; 18) uterus, 19) control reaction without cDNA template. Fertility and Sterility 2010 93, 2507-2512DOI: (10.1016/j.fertnstert.2009.08.051) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Relative quantification of poly(A)-binding protein nuclear-like 1 (Pabpnl1) and methyl-CpG–binding domain protein 3–like 2 (Mbd3l2) transcripts. (A) Pabpnl1 expression in oocytes before maturation with germinal vesicle (GV), after in vitro maturation at metaphase II (MII) stage of high-quality (A) and low-quality (B) oocytes; ND=transcripts not detected. (B) Mbd3l2 expression in early bovine embryos. The vertical lines are the standard error; different letters above the numbers indicate statistical significance (P<.05). Fertility and Sterility 2010 93, 2507-2512DOI: (10.1016/j.fertnstert.2009.08.051) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions