Volume 6, Issue 1, Pages 41-44 (April 1999) Effects of sodium hyaluronic acid on fibrinolytic factors in the synovial fluid (in vivo)  Tohgo Nonaka, Hiraku Kikuchi, Wataru Shimada, Hiroaki Itagane, Terumasa Ikeda, Chiaki Hamanishi, Seisuke Tanaka  Pathophysiology  Volume 6, Issue 1, Pages 41-44 (April 1999) DOI: 10.1016/S0928-4680(98)00031-5

Fig. 1 The activity of PA in synovial fluid. Electrophoretic enzymography of synovial fluid collected from osteoarthritis deformans of the knee. The gel was incubated with 0.1 M glycine–NaOH buffer pH 8.3 at 37°C for 48 h. Lane 1, t-PA; lane 2, u-PA; lane 3, synovial fluid; lane 4, synovial fluid treated with anti-t-PA IgG; lane 5, synovial fluid treated with anti-u-PA IgG. Pathophysiology 1999 6, 41-44DOI: (10.1016/S0928-4680(98)00031-5)

Fig. 2 Level of u-PA activity on administration with HA (a typical case of improvement). Synovial fluid (3 ml) was collected prior to the introduction of HA and the total synovial fluid was collected after 3 h. This procedure was repeated over a 4-week period. The collected synovial fluid was centrifuged for 5 min at 3000 rpm and the cellular components were discarded. Lane 1, t-PA; lane 2, u-PA; lane 3, synovial fluid for the week prior to the introduction of HA; lane 4, synovial fluid from the 1st week after the introduction of HA; lane 5, synovial fluid from the 2nd week after the introduction of HA; lane 6, synovial fluid from 3rd week after the introduction of HA; lane 7, synovial fluid from the 4th week after the introduction of HA. Pathophysiology 1999 6, 41-44DOI: (10.1016/S0928-4680(98)00031-5)

Fig. 3 PAI-1/u-PA ratio among the individual patients were observed from the first HA dose through to the 4th week in nine out of 12 patients who showed an improvement: •, improvement; ■, no improvement. Pathophysiology 1999 6, 41-44DOI: (10.1016/S0928-4680(98)00031-5)