Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies Laszlo.

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Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies Laszlo Óvári, M.D., Leyla Sati, M.S., Jill Stronk, M.S., Antal Borsos, M.D., David C. Ward, Ph.D., Gabor Huszar, M.D. Fertility and Sterility Volume 93, Issue 7, Pages 2255-2261 (May 2010) DOI: 10.1016/j.fertnstert.2009.05.033 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Panels of double treated spermatozoa with aniline blue staining (left) and FISH (right). Arrows on left side indicate staining intensity patterns. Fertility and Sterility 2010 93, 2255-2261DOI: (10.1016/j.fertnstert.2009.05.033) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Relationship between proportion of sperm with intermediate aniline blue staining and disomy frequencies (r = 0.6, P = .002). Fertility and Sterility 2010 93, 2255-2261DOI: (10.1016/j.fertnstert.2009.05.033) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions