Development and validation of an accurate quantitative real-time polymerase chain reaction–based assay for human blastocyst comprehensive chromosomal.

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Presentation transcript:

Development and validation of an accurate quantitative real-time polymerase chain reaction–based assay for human blastocyst comprehensive chromosomal aneuploidy screening Nathan R. Treff, Ph.D., Xin Tao, M.Sc., Kathleen M. Ferry, B.Sc., Jing Su, M.Sc., Deanne Taylor, Ph.D., Richard T. Scott, M.D., H.C.L.D. Fertility and Sterility Volume 97, Issue 4, Pages 819-824.e2 (April 2012) DOI: 10.1016/j.fertnstert.2012.01.115 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Examples of qPCR-based 24-chromosome copy number results from 5-cell samples derived from nine cell lines with previously well characterized karyotypes. Fertility and Sterility 2012 97, 819-824.e2DOI: (10.1016/j.fertnstert.2012.01.115) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Box-whisker plots representing the distribution of average 24-chromosome four-loci copy number standard deviations for each of the 42 cell line samples and 71 blastocyst biopsies. For each sample type, one outlier was identified, including the only cell line sample with an inconsistent qPCR diagnosis. Fertility and Sterility 2012 97, 819-824.e2DOI: (10.1016/j.fertnstert.2012.01.115) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Examples of (gray) single-nucleotide polymorphism microarray– and (white) qPCR-based 24-chromosome copy number results from blastocyst-stage embryo biopsies. Fertility and Sterility 2012 97, 819-824.e2DOI: (10.1016/j.fertnstert.2012.01.115) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions