rSema4A does not enhance Treg cell suppressive activity in vitro.

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rSema4A does not enhance Treg cell suppressive activity in vitro. rSema4A does not enhance Treg cell suppressive activity in vitro. CD4+CD25depleted T cells (Tresp) cells were labeled with 5 μM CFSE and then stimulated with CD3/CD28 T cell activator when cocultured with decreasing numbers of CD4+CD25+CD127low Treg cells in the presence of rhSema4A. (A) shows representative CFSE histograms for Tresp cell proliferation where each cell division cycle is clearly distinguished. (B) shows various overlays of data for Tresp cell proliferation in triplicates of each culture setting from two experiments. (C) shows a median percent suppression of Tresp cell proliferation by Treg cells for each Treg cell:Tresp cell combination. *p < 0.035 for each of five cell generations, Tresp cell versus Treg cell:Tresp cell 1:1. ** and ***p < 0.05 for five cell generations, Treg cell:Tresp cell 1:1 and 1:9 versus Treg cell:Tresp cell 1:1 and 1:9 plus rSema4A. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors