Volume 6, Issue 3, Pages 665-674 (May 2013) Rice MtN3/Saliva/SWEET Family Genes and Their Homologs in Cellular Organisms  Meng Yuan, Shiping Wang  Molecular Plant  Volume 6, Issue 3, Pages 665-674 (May 2013) DOI: 10.1093/mp/sst035 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 1 Structures of MtN3/Saliva/SWEET-Type Proteins. According to the number of MtN3-saliva domains in a protein, MtN3/saliva/SWEET-type proteins can be classified into two types: those harboring two MtN3/saliva domains (shown with red and blue colors), such as rice Xa13/Os8N3/OsSWEET11 protein, and those harboring only one MtN3/saliva domain (shown with red color), such as Methanocaldococcus jannaschii NP_247074 protein. The topology of Xa13/Os8N3/OsSWEET11 protein is determined by analyzing a set of truncated proteins using a split-ubiquitin system for integral membrane protein (Yuan et al., 2010). The topology of NP_247074 protein is from the Transporter Classification Database (TCDB, www.tcdb.org). Molecular Plant 2013 6, 665-674DOI: (10.1093/mp/sst035) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 2 Phylogenetic Relationship of MtN3/Saliva/SWEET Family Proteins in Different Species. The numbers of MtN3/saliva domain in each protein were predicted by searching the Conserved Domain Database (www.ncbi.nlm.nih.gov/cdd) using the full-length of amino acid sequence. Sequences were analyzed by the neighbor-joining method with genetic distance calculated by MEGA5.1 (www.megasoftware.net/). The numbers at the nodes represent bootstrap percentage values based on 1000 replications. The black five-pointed star represents the number of MtN3/saliva domains in each protein. Molecular Plant 2013 6, 665-674DOI: (10.1093/mp/sst035) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 3 Rice Xa13/Os8N3/OsSWEET11 Is Involved in the Regulation of Reproductive Development and Rice–Bacterium Interaction. ASuppressing Xa13/Os8N3/OsSWEET11 by RNA interference (RNAi) resulted in almost complete sterile of the transgenic plant. BSuppressing Xa13/Os8N3/OsSWEET11 enhanced rice resistance to Xoo strain PXO99. Molecular Plant 2013 6, 665-674DOI: (10.1093/mp/sst035) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 4 Hierarchical Cluster of Expression Profiles from Microarray Data of Rice MtN3/Saliva/SWEET-Type Genes in 28 Tissues Covering the Whole Life Cycle of Rice Varieties Minghui 63 (A) and Zhenshan 97 (B). Data were generated from the microarray database (www.ncbi.nlm.nih.gov/; accession number GSE19024; Wang et al., 2010). Plumule 1, plumule at 48 h after emergence under light; Plumule 2, plumule at 48 h after emergence in the dark; Shoot at 2 tiller, shoot of seedling with two tillers; Leaf 1, leaf at secondary-branch primordium stage; Leaf 2, leaf at 4–5-cm young panicle stage; Flag leaf 1, flag leaf at 5 d before heading; Flag leaf 2, flag leaf at 14 d after heading; Sheath 1, sheath at secondary-branch primordium stage; Sheath 2, sheath at 4–5-cm young panicle stage; Stem 1, stem at 5 d before heading; Stem 2, stem at heading stage; Embryo & radicle, embryo and radicle after germination; Leaf and root at 3, leaf and root at three-leaf stage; Radicle 1, radicle at 48 h after emergence under light; Radicle 2, radicle at 48 h after emergence in the dark; Root at 2 tiller, root of seedling with two tillers; Panicle 1, panicle at secondary-branch primordium stage; Panicle 2, panicle at pistil and stamen primordium differentiation stage; Panicle 3, panicle at pollen-mother cell formation stage; Panicle 4, panicle at 4–5-cm young panicle stage; Panicle 5, panicle at heading stage; Stamen, stamen at 1 d before flowering; Spikelet, spikelet at 3 d after pollination; Hull, hull at 1 d before flowering; Endosperm 1, endosperm at 14 d after heading; Endosperm 2, endosperm at 7 d after pollination; Endosperm 3, endosperm at 21 d after pollination; Geminating seed, germinating seed at 72 h of imbibition. The color key is the log2 transformation of the average signal values. Green and red denote low and high level of expression, respectively. Molecular Plant 2013 6, 665-674DOI: (10.1093/mp/sst035) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions