Volume 2, Issue 1, Pages (January 2009)

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Volume 2, Issue 1, Pages 108-119 (January 2009) ABA-Mediated Inhibition of Germination Is Related to the Inhibition of Genes Encoding Cell-Wall Biosynthetic and Architecture: Modifying Enzymes and Structural Proteins in Medicago truncatula Embryo Axis  Gimeno-Gilles Christine , Lelièvre Eric , Viau Laure , Malik-Ghulam Mustafa , Ricoult Claudie , Niebel Andreas , Leduc Nathalie , Limami Anis M.   Molecular Plant  Volume 2, Issue 1, Pages 108-119 (January 2009) DOI: 10.1093/mp/ssn092 Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 1 Effect of Permanent and Transient Abscisic Acid Treatment on Medicago truncatula Seed Germination and Radicle Growth. Germination rates (A) and radicle length (B) are the mean of 150 measurements taken on 150 seeds germinated on water, 100 μM ABA or 100 μM ABA during 15 h before transfer of the seeds on water. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 2 Microscopic Observation of Longitudinal Sections of Medicago truncatula Embryos at Two Developmental Stages. Imbibed seed (5 h) before germination completion (A) and after germination completion and radicle emergence (21 h) (B). ×4 enlargement shows details of cells clustered in spindles. Boxes correspond to the apical region (1) and the upper region (2) of the radicle. Int, internal cell layers; Ext, external cell layers; CP, cortical parenchyma; V, vascular tissues; RC, root cap. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 3 Changes in Cortical Parenchyma Cell Length (A) and Anatomy (B) in the Apical and Upper Region of Medicago truncatula Radicle at Various Developmental Stages, Before and After Germination Completion. Pictures are presented on highly saturated inverted colors. ×40. CP, cortical parenchyma; RC, root cap. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 4 Effect of 10 μM Oryzalin on Medicago truncatula Seed Germination and Seedling Post-Germination Growth. O, oryzalin condition; W, water condition. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 5 Microscopic Observation of a Longitudinal Section of Medicago truncatula Embryo Axis Cells after Germination Completion and Radicle Emergence after 25 h of Oryzalyn (10 μm) Treatment. Hypocotyl (A), upper region (B), sub-apical region (C), and apical region (D) of the radicle. Pictures are presented on highly saturated inverted colors. ×40. RC, root cap; CP, cortical parenchyma; E, epidermis. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 6 Expression of Genes Involved in Cell-wall Biosynthesis and Architecture Modification and Cell Cycle in Medicago truncatula Embryo Axis under Various Conditions of Germination: Water, Permanent, and Transient (100 μM) ABA and 10 μM Oryzalin Treatment. The results are the mean ± SE of at least two biological replicates with duplicated PCR reaction for determining Ct values. Expression levels are presented in arbitrary units. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

Figure 6 Expression of Genes Involved in Cell-wall Biosynthesis and Architecture Modification and Cell Cycle in Medicago truncatula Embryo Axis under Various Conditions of Germination: Water, Permanent, and Transient (100 μM) ABA and 10 μM Oryzalin Treatment. The results are the mean ± SE of at least two biological replicates with duplicated PCR reaction for determining Ct values. Expression levels are presented in arbitrary units. Molecular Plant 2009 2, 108-119DOI: (10.1093/mp/ssn092) Copyright © 2009 The Authors. All rights reserved. Terms and Conditions