Alopecia areata profiling shows TH1, TH2, and IL-23 cytokine activation without parallel TH17/TH22 skewing Mayte Suárez-Fariñas, PhD, Benjamin Ungar,

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Presentation transcript:

Alopecia areata profiling shows TH1, TH2, and IL-23 cytokine activation without parallel TH17/TH22 skewing Mayte Suárez-Fariñas, PhD, Benjamin Ungar, BA, Shinji Noda, MD, PhD, Anjali Shroff, MD, Yasaman Mansouri, MD, Judilyn Fuentes- Duculan, MD, Annette Czernik, MD, Xiuzhong Zheng, MSc, Yeriel D. Estrada, MSc, Hui Xu, MSc, Xiangyu Peng, MSc, Avner Shemer, MD, James G. Krueger, MD, PhD, Mark G. Lebwohl, MD, Emma Guttman-Yassky, MD, PhD Journal of Allergy and Clinical Immunology Volume 136, Issue 5, Pages 1277-1287 (November 2015) DOI: 10.1016/j.jaci.2015.06.032 Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, Representative clinical images of limited (≤25%) and extensive (>25%) scalp involvement and total hair loss in patients with AA. B, A principal component analysis (PCA) based on microarrays shows separate groupings of lesional (LS), nonlesional (NL), and healthy scalp mRNA expressions. Five lesional samples from GSE45512 (in red) grouped with the other lesional samples. Journal of Allergy and Clinical Immunology 2015 136, 1277-1287DOI: (10.1016/j.jaci.2015.06.032) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Heat maps of immune (A) and keratin-associated (B) DEG subsets. Samples are grouped by extent of AA involvement (AU/AT is darkest): red, higher expression; blue, lower expression. Group comparisons (FCHs) are indicated. +False discovery rate adjusted P value < .1; *false discovery rate adjusted P value < .05; and **false discovery rate adjusted P value < .01. LS, Lesional; N, normal; NL, nonlesional. Journal of Allergy and Clinical Immunology 2015 136, 1277-1287DOI: (10.1016/j.jaci.2015.06.032) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Real-time RT-PCR comparisons of inflammatory markers in respective lesional (LS), nonlesional (NL), and healthy scalp and skin samples from patients with AA, AD, or psoriasis (pso) and healthy subjects. hARP, Human acidic ribosomal protein. Mean (log2 expression/hARP) ± SEM. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology 2015 136, 1277-1287DOI: (10.1016/j.jaci.2015.06.032) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 A, Venn diagram of upregulated (red) and downregulated (blue) DEGs in HI versus whole-group AA. Sizes of circles are proportional to numbers of DEGs. B, A heat map illustrating an immune subset comparison of patients with HI and MI AA. Healthy, nonlesional (NL), and lesional (LS) scalp data are compared. FCHs between indicated comparisons within the HI group are presented. +False discovery rate adjusted P value < .1; *false discovery rate adjusted P value < .05; and **false discovery rate adjusted P value < .01. Journal of Allergy and Clinical Immunology 2015 136, 1277-1287DOI: (10.1016/j.jaci.2015.06.032) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 A, Heat map illustrating immune (A) and keratin-associated (B) DEG subsets between patients with limited (≤25%) and extensive AA (>25%) involvement are shown. FCHs between indicated groups are presented. Box plots of immune (C) and keratin-associated (D) DEGs of lesional (LS) and nonlesional (NL; vs healthy) scalp samples are depicted for both groups (median FCH vs healthy scalp, boxes show 25% to 75% ± whiskers 95%; 0 = normal). +False discovery rate adjusted P value < .1; *false discovery rate adjusted P value < .05; and **false discovery rate adjusted P value < .01. Journal of Allergy and Clinical Immunology 2015 136, 1277-1287DOI: (10.1016/j.jaci.2015.06.032) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions