2014 GEM Midwest Trial Results & New Releases

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2014 GEM Midwest Trial Results & New Releases Germplasm Enhancement of Maize C. Gardner, F. Engstrom, M. Peters, M. Shen USDA-ARS NCRPIS GEM - December 10, 2014

Commercial Checks 2014 Germplasm Enhancement of Maize Midwest RM Hybrid Midwest RM HC33xLH287 109 Pioneer 34R65 109 MBS3520 105-115 hybrids MBS8814 107-117 hybrids MBS3520/MBS8814 112 Pioneer 33F85 114 LH200xLH262 118 Pioneer 31D58 119 Germplasm Enhancement of Maize

2015 GEM Releases from Ames GEM Code Pedigree Race Type Het Grp GEMN-0260 (GEMN-0140/GEMN-0097)-B-B-026-B-B Dente Amarillo/FS8BT 12.5% Tropical NS GEMN-0261 (GEMN-0140/GEMN-0097)-B-B-027-B-B GEMS-0262 (GEMS-0147/GEMS-0180)-B-104-001-B-B Suwan/Tusón SS GEMS-0263 BR105:S1626(GEMS-0175)-B-049-B-B Composite Suwan/Tusón GEMS-0264 BR105:S1643-078-001-B-B Composite Suwan 25% Tropical GEMS-0265 DKXL212:S0950-B-083-B-B Hybrid Tropical GEMN-0266 NEI9008:N0826(GEMN-0155)-B-074-B-B Suwan/Cristalino Colorado GEMN-0267 NEI9008:N0826(GEMN-0155)-B-095-B-B GEMS-0268 NS1:S0852-B-021-B-B Suwan GEMS-0269 NS1:S0852-B-054-B-B

GEMN-0260 (GEMN-0140/GEMN-0097)-B-B-026 145402 Tester: HC33 145403 Tester: MBS3520 GEM Code Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL % Yd /CHK GEMN-0260 (GEMN-0140/GEMN-0097)-B-B-026 17 183 20.0 9.1 55.0 1.2 7.8 3 161 89.2 18.5 8.8 53.5 2.4 3.0 Test Entry Means 157 19.2 8.2 55.9 4.4 20.4 7.9 54.2 3.1 9.7 Check Means 191 21.1 9.0 7.0 181 21.8 8.3 55.2 1.9 4.9 Overall Means 19.5 4.7 164 20.6 8.0 54.4 CV 14 13 6.6 F-value 5.0 7.2 6.0 LSD (p=0.05) 26.4 1.6 23 1.5 Germplasm Enhancement of Maize Better roots than GEMN-0261;Dente Amarelo (Brazil) x Mixed;

GEMN-0261 (GEMN-0140/GEMN-0097)-B-B-027 145402 Tester: HC33 145403 Tester: MBS3520 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL (GEMN-0140/GEMN-0097)-B-B-027 16 175 19.4 9.0 55.0 0.4 2.2 4 183 20.0 9.2 53.9 1.9 21.2 Test Entry Means 157 19.2 8.2 55.9 2.4 4.4 161 20.4 7.9 54.2 3.1 9.7 Check Means 191 21.1 7.0 181 21.8 8.3 55.2 4.9 Overall Means 19.5 4.7 164 20.6 8.0 54.4 3.0 CV 14.2 13.1 6.6 F-value 5.0 7.2 6.0 LSD (p=0.05) 26.4 1.6 23.6 1.5 Germplasm Enhancement of Maize Hybrid Tropical – Brazil; more consistent yield than GEMN-0260

GEMS-0262 (GEMS-0147/GEMS-0180)-B-104-001 145401 Tester: LH287 145404 Tester MBS8814 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL (GEMS-0147/GEMS-0180)-B-104-001 29 202 24.0 8.5 52.6 0.8 8.1 12 198 25.1 7.9 51.6 0.6 3.9 Test Entry Means 179 23.2 7.7 53.3 3.5 10.8 183 23.3 53.7 1.4 4.9 Check Means 194 22.2 8.8 55.9 5.1 196 22.6 8.7 55.3 1.8 10.1 Overall Means 180 23.1 7.8 53.6 3.3 10.2 184 8.0 53.9 5.4 CV 9.3 4.3 6.3 F-value 3.2 10.4 2.2 LSD (p=0.05) 20.4 1.2 21.5 1.6 Germplasm Enhancement of Maize Thai tropical inbred x Tuson (Guatemala)

GEMS-0263 BR105:S1626(GEMS-0175)-B-049 Germplasm Enhancement of Maize 121403 Tester: MBS9508 141408 Tester: LH287 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL BR105:S1626(GEMS-0175)-B-049 43 158 21.4 7.4 58.8 0.4 8.1 14 202 24.6 8.2 53.4 2.1 4.3 Test Entry Means 135 22.0 6.2 58.2 4.6 10.3 186 23.3 8.0 1.9 7.8 Check Means 134 21.5 6.3 56.9 1.0 8.5 198 22.1 9.0 56.3 1.6 2.9 Overall Means 187 23.2 53.7 CV 15.7 9.2 F-value 1.5 8.3 2.8 15.3 LSD (p=0.05) 30.4 1.3 17.2 Germplasm Enhancement of Maize Composite Suwan (Brazil) x Tuson (Br. Virgin Islands)

GEMS-0264 BR105:S1643-078-001 Germplasm Enhancement of Maize 121304 Tester: AGR-nSS 141408 Tester: LH287 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL BR105:S1643-078-001 43 169.2 20.5 8.3 56.3 0.0 9.4 36 204.3 24.7 52.4 1.4 7.9 Test Entry Means 151.5 21.1 7.2 55.6 1.0 186.2 23.3 8.0 53.4 1.9 7.8 Check Means 162.1 19.6 56.2 0.2 3.3 198.9 22.1 9.0 1.6 2.9 Overall Means 187.3 23.2 8.1 53.7 7.4 CV 15.1 6.9 9.2 4.3 F-value 2.4 4.1 2.8 15.3 LSD (p=0.05) 29.7 1.8 17.2 Germplasm Enhancement of Maize Suwan (Thailand); top of test with LH287 tester; equal maturity to LH200/LH262 check; much shorter ear ht; better roots than others in this family

GEMS-0265 DKXL212:S0950-B-083 Germplasm Enhancement of Maize 141408 Tester: LH287 141413 Tester: MBS8814 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL DKXL212:S0950-B-083 32 196 22.3 8.8 54.7 1.2 2.6 189 22.8 8.3 52.4 1.4 0.5 Test Entry Means 186 23.3 8.0 53.4 1.9 7.8 180 22.7 51.9 1.3 4.0 Check Means 198 22.1 9.0 56.3 1.6 2.9 21.4 8.7 54.1 1.7 2.7 Overall Means 187 23.2 8.1 53.7 7.4 181 22.6 52.1 3.9 CV 9.2 4.3 8.2 5.7 F-value 2.8 15.3 3.2 10.5 LSD (p=0.05) 17.2 1.0 14.9 Germplasm Enhancement of Maize MBS8815 tester in previous year also had an excellent record. Hybrid tropical from Brazil. Equal in maturity to 33F85

GEMN-0266 NEI9008:N0826(GEMN-0155)-B-074 141411 Tester: HC33 141412 Tester: MBS3520 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL NEI9008:N0826(GEMN-0155)-B-074 13 179 20.3 8.9 54.3 3.3 13.3 11 201 24.2 8.3 53.4 1.8 9.8 Test Entry Means 156 19.8 7.9 54.9 3.7 6.0 172 22.3 7.7 53.9 2.0 9.0 Check Means 170 20.2 8.4 55.3 1.5 4.1 186 21.9 8.5 55.1 1.1 Overall Means 157 19.9 55.0 3.4 5.7 174 7.8 54.1 1.9 CV 9.9 5.1 11.6 6.2 F-value 4.3 14.9 4.6 14.2 LSD (p=0.05) 16.8 20.9 1.4 Germplasm Enhancement of Maize Suwan (Thai) x Cristalino Colorado (Argentina); top of test, low variance;

GEMN-0267 NEI9008:N0826(GEMN-0155)-B-095 141411 Tester: HC33 141412 Tester: MBS3520 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL NEI9008:N0826(GEMN-0155)-B-095 16 177 19.7 9.0 54.6 6.3 7.8 14 196 21.1 9.3 53.3 4.0 8.2 Test Entry Means 156 19.8 7.9 54.9 3.7 6.0 172 22.3 7.7 53.9 2.0 Check Means 170 20.2 8.4 55.3 1.5 4.1 186 21.9 8.5 55.1 1.1 Overall Means 157 19.9 55.0 3.4 5.7 174 54.1 1.9 CV 9.9 5.1 11.6 6.2 F-value 4.3 14.9 4.6 14.2 LSD (p=0.05) 16.8 20.9 1.4 Germplasm Enhancement of Maize Suwan (Thai) x Cristalino Colorado (Argentina); 31D58 maturity; top of test, low variance ***

GEMS-0268 NS1:S0852-B-021 121307 Tester: MON-nSS 141413 Tester: MBS8814 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL NS1:S0852-B-021 19 175 20.5 8.6 56.3 5.7 3.5 16 205 23.8 8.7 49.1 3.2 11.5 Test Entry Means 143 18.9 7.6 58.1 16.6 0.5 180 22.7 8.0 51.9 1.3 4.0 Check Means 154 20.9 7.4 58.2 0.2 186 21.4 54.1 1.7 2.7 Overall Means 181 22.6 52.1 3.9 CV 14.8 6.9 8.2 F-value 1.5 4.9 10.5 LSD (p=0.05) 29.7 2.0 14.9 LH287 tester in 2014 also; 199 bu, Y/M-8.1; Suwan (thailand); watch roots, but great yield and very large perse ears; moderate plant & ear ht; white cobs, good grain quality

GEMS-0269 NS1:S0852-B-054 Germplasm Enhancement of Maize 141408 Tester: LH287 141413 Tester: MBS8814 Pedigree Ent Yield B/A % Moist Y/M TWT % SL % RL NS1:S0852-B-054 21 187.6 22.2 8.6 52.5 2.1 9.65 197.2 21.9 9.0 51.7 0.9 9.5 Test Entry Means 186.2 23.3 8.0 53.4 1.9 7.8 180.5 22.7 51.9 1.3 4.0 Check Means 198.9 22.1 56.3 1.6 2.9 186.1 21.4 8.7 54.1 1.7 2.7 Overall Means 187.3 23.2 8.1 53.7 7.4 181.0 22.6 52.1 3.9 CV 9.2 4.3 8.2 5.7 F-value 2.8 15.3 3.2 10.5 LSD (p=0.05) 17.2 1.0 14.9 Germplasm Enhancement of Maize Suwan (Thailand); high yielding, early line; high variance in hybrid combination

Top Cross Estimated Relative Maturity Average GEM Code Pedigree Estimated RM Ear Height (cm) Hybrid Checks GEMN-0260 (GEMN-0140/GEMN-0097)-B-B-026 110 HC33xLH287 109 GEMN-0261 (GEMN-0140/GEMN-0097)-B-B-027 90 MBS3633xMBS8814 113 125 GEMS-0262 (GEMS-0147/GEMS-0180)-B-104-001 119 100 LH200xLH262 118 140 GEMS-0263 BR105:S1626(GEMS-0175)-B-049 116 34R65 ~109 105 GEMS-0264 BR105:S1643-078-001 33F85 ~114 GEMS-0265 DKXL212:S0950-B-083 31D58 ~119 GEMN-0266 NEI9008:N0826(GEMN-0155)-B-074 130 GEMN-0267 NEI9008:N0826(GEMN-0155)-B-095 115 120 GEMS-0268 NS1:S0852-B-021 GEMS-0269 NS1:S0852-B-054 95

Above Average Resistance GLS & Goss’s Wilt Above Average Resistance GLS GEMS-0263 GEMS-0265 GEMS-0268 Goss’s Wilt GEMS-0269 Germplasm Enhancement of Maize 2nd Goss’s Release 34 Lines All Years Based on ratings from J. Dodd, PSR

Above Average Resistance FUSER & Head Smut Above Average Resistance Germplasm Enhancement of Maize FUSER GEMS-0264 GEMN-0266 GEMN-0267 Head Smut GEMS-0268 39 Lines All Years 47 Lines All Years Based on ratings from M. Mancl, DuPont Pioneer (Woodland, CA)

Southern Leaf Blight Resistance (SLB) Above Average Resistance GEMS-0223 GEMN-0239 Germplasm Enhancement of Maize 87 Lines All Years Based on ratings from P. Balint-Kurti, USDA-ARS PSRU

Questions? Thanks to all Germplasm Enhancement Cooperators for their contributions and efforts. We sincerely hope that your role in the GEM Project has a positive impact on your program. Please share your thoughts and ideas as to how GEM can better accomplish diversifying the genetic base of corn production and reduce crop vulnerability.

Thoughts as GEM transitions New GEM Coordinator/Maize Geneticist needed in Ames Experience with and passion for germplasm introgression Superior communication and collaborative skills Skill in use of statistical and IM tools relevant to plant breeding Staff needs better analytical tools and to import data into database more readily. Germplasm performance information needs to be publicly accessible via a database to supports user queries and information retrieval. Update as data becomes available. GEM needs to leverage US and international network capacities to better evaluate and characterize germplasm in order to deploy appropriate genetic resources to address production challenges.

Thoughts continued… *Cycle time must be reduced. This requires more resources , either internal or via collabors. *Germplasm Access is becoming more challenging not less. Need to access unencumbered, valuable germplasm. *Phytosanitary requirements for germplasm exchange are becoming more rigorous and more testing is required. *AP testing and declarations may be required for exchange. * Technical and managerial skills required for project execution are every bit as rigorous as for a commercial breeder but performed in an environment that is both federal and academic.

Best wishes to all in 2015!