Volume 52, Issue 6, Pages 1736-1743 (December 2007) Functional and Histological Changes after Myoblast Injections in the Porcine Rhabdosphincter  Michael Mitterberger, Germar M. Pinggera, Rainer Marksteiner, Eva Margreiter, Raffael Plattner, Günter Klima, Georg Bartsch, Hannes Strasser  European Urology  Volume 52, Issue 6, Pages 1736-1743 (December 2007) DOI: 10.1016/j.eururo.2007.05.007 Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 1 (a,b) Example of a pressure curve before and after implantation of myoblasts. Arrow 1 shows preoperative peak. Arrow 2 shows injection site 2. Arrow 3 shows injection site 1. The postoperative peak is the same as the preoperative peak. At the site of injection of myoblasts (arrows 2 and 3), the urethral closure pressures have dramatically increased after injection of cells. European Urology 2007 52, 1736-1743DOI: (10.1016/j.eururo.2007.05.007) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 2 (a) Fluorescence microscopy following injection of myoblasts into a procine rhabdosphincter. The myoblasts have been labelled with PKH prior to injection. 3 wk after implantation of 2.6×107 myoblasts the injected cells have formed new myotubes. (Original magnification: ×20.) (b) Corresponding histological section to Fig. 3; trichrome Masson-Goldner staining. The newly formed myofibres in the porcine rhabdosphincter can be clearly visualised (marked with arrow). In addition, no signs of inflammation or “bulks” of tissue can be seen. (Original magnification: ×20.) (c) Fluorescence microscopy following injection of myoblasts into a porcine rhabdosphincter. The myoblasts have been labelled with PKH prior to injection. Three weeks after implantation of 2.6×107 myoblasts, the injected cells have formed new myotubes. (Original magnification: ×40.) (d) Corresponding histological section to Fig. 3; trichrome Masson-Goldner staining. The newly formed myofibres in the porcine rhabdosphincter can be clearly visualised (marked with arrow). In addition, no signs of inflammation or “bulks” of tissue can be seen (marked with arrow). (Original magnification: ×20.) European Urology 2007 52, 1736-1743DOI: (10.1016/j.eururo.2007.05.007) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 3 Histological section of the urethra showing rhabdospincter cells of a similar area without implanted myoblasts. (Original magnification: ×20.) European Urology 2007 52, 1736-1743DOI: (10.1016/j.eururo.2007.05.007) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 4 Pre/postoperative urethral pressure profile (UPP) values at the injection sites expressed in whole numbers (in cm H2O). European Urology 2007 52, 1736-1743DOI: (10.1016/j.eururo.2007.05.007) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 5 Difference in pressure (pre/postoperative) at the injection sites expressed in whole numbers (in cm H2O). European Urology 2007 52, 1736-1743DOI: (10.1016/j.eururo.2007.05.007) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 6 Clonal myoblast cell culture of pig with antidesmin antibody labelling. (Original magnification: ×20.) European Urology 2007 52, 1736-1743DOI: (10.1016/j.eururo.2007.05.007) Copyright © 2007 European Association of Urology Terms and Conditions