Enzymes Reaction Part II

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Enzyme kinetics -- Michaelis Menten kinetics

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Presentation transcript:

Enzymes Reaction Part II PSSH Enzyme Kinetics

Active Intermediates/Free Radicals

Active Intermediates/Free Radicals

Active Intermediates/Free Radicals Mechanism:

Active Intermediates/Free Radicals Mechanism:

Active Intermediates/Free Radicals Mechanism:

Active Intermediates/Free Radicals Mechanism:

Pseudo Steady State Hypothesis (PSSH) A* is an active intermediate that is highly reactive. It disappears as fast as it is formed.

Pseudo Steady State Hypothesis (PSSH) A* is an active intermediate that is highly reactive. It disappears as fast as it is formed.

Pseudo Steady State Hypothesis (PSSH) A* is an active intermediate that is highly reactive. It disappears as fast as it is formed.

Pseudo Steady State Hypothesis (PSSH) A* is an active intermediate that is highly reactive. It disappears as fast as it is formed.

Pseudo Steady State Hypothesis (PSSH) A* is an active intermediate that is highly reactive. It disappears as fast as it is formed.

Pseudo Steady State Hypothesis (PSSH) A* is an active intermediate that is highly reactive. It disappears as fast as it is formed.

Pseudo Steady State Hypothesis (PSSH)

Enzymes: Michaelis-Menten Kinetics

Enzymes: Michaelis-Menten Kinetics

Enzymes: Michaelis-Menten Kinetics

Urea Decomposition A given enzyme can only catalyze only one reaction. Urea is decomposed by the enzyme urease, as shown below.

Urea Decomposition A given enzyme can only catalyze only one reaction. Urea is decomposed by the enzyme urease, as shown below.

Urea Decomposition A given enzyme can only catalyze only one reaction. Urea is decomposed by the enzyme urease, as shown below. Rate Laws:

Urea Decomposition A given enzyme can only catalyze only one reaction. Urea is decomposed by the enzyme urease, as shown below. Rate Laws:

Urea Decomposition A given enzyme can only catalyze only one reaction. Urea is decomposed by the enzyme urease, as shown below. Rate Laws: If

Urea Decomposition

Urea Decomposition

Urea Decomposition

Urea Decomposition

Urea Decomposition VMAX rP Km S

Urea Decomposition

Types of Enzyme Inhibition

Types of Enzyme Inhibition

Types of Enzyme Inhibition

Types of Enzyme Inhibition

Types of Enzyme Inhibition