Volume 114, Issue 2, Pages 295-304 (February 1998) Genetic analysis of long-term Barrett's esophagus epithelial cultures exhibiting cytogenetic and ploidy abnormalities  M.Corinna Palanca-Wessels, Michael T. Barrett, Patricia C. Galipeau, Katherine L. Rohrer, Brian J. Reid, Peter S. Rabinovitch  Gastroenterology  Volume 114, Issue 2, Pages 295-304 (February 1998) DOI: 10.1016/S0016-5085(98)70480-9 Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 Phase-contrast microscopy of Barrett's epithelial cell cultures. The monolayer cultures show a cobblestone-like morphology at confluency that is typical of epithelial cells. (A) A primary culture showing cells explanting from pieces of tissue after 7 days in culture; this particular culture was eventually overgrown by fibroblasts subsequent to the first passage. (B) A representative long-term Barrett's epithelial culture, CP-94251, at passage 8. Gastroenterology 1998 114, 295-304DOI: (10.1016/S0016-5085(98)70480-9) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 Phase-contrast microscopy of Barrett's epithelial cell cultures. The monolayer cultures show a cobblestone-like morphology at confluency that is typical of epithelial cells. (A) A primary culture showing cells explanting from pieces of tissue after 7 days in culture; this particular culture was eventually overgrown by fibroblasts subsequent to the first passage. (B) A representative long-term Barrett's epithelial culture, CP-94251, at passage 8. Gastroenterology 1998 114, 295-304DOI: (10.1016/S0016-5085(98)70480-9) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 2 Flow-cytometric analyses of asynchronous and 4N sorted Barrett's epithelial cultures. Cell cycle distribution of asynchronous cells is shown in A–D. Note the elevated fraction of cells with G2/tetraploid (4N) DNA content. (A) CP-52731, (B) CP-18821, (C) CP-94251, and (D) KR-42421. DNA histograms of 4N sorted cells from one of the Barrett's epithelial cultures, CP-18821, are shown in E and F. Cells (4N sorted) from CP-18821 were returned to culture and harvested 72 hours later. Sorted 4N cells: E, immediately after sort; and F, 72 hours later. Gastroenterology 1998 114, 295-304DOI: (10.1016/S0016-5085(98)70480-9) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 3 Karyotypes of a (A) near-diploid cell and (B) near-tetraploid cell from culture CP-52731. Clonal abnormalities found in the near-diploid cell that are also seen in the near-tetraploid cell include a translocation onto the 9p chromosome, a 17p abnormality, and an unidentified marker chromosome. Gastroenterology 1998 114, 295-304DOI: (10.1016/S0016-5085(98)70480-9) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 3 Karyotypes of a (A) near-diploid cell and (B) near-tetraploid cell from culture CP-52731. Clonal abnormalities found in the near-diploid cell that are also seen in the near-tetraploid cell include a translocation onto the 9p chromosome, a 17p abnormality, and an unidentified marker chromosome. Gastroenterology 1998 114, 295-304DOI: (10.1016/S0016-5085(98)70480-9) Copyright © 1998 American Gastroenterological Association Terms and Conditions