Intratumoral Patterns of Clonal Evolution in Meningiomas as Defined by Multicolor Interphase Fluorescence in Situ Hybridization (FISH) José María Sayagués,

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Intratumoral Patterns of Clonal Evolution in Meningiomas as Defined by Multicolor Interphase Fluorescence in Situ Hybridization (FISH) José María Sayagués, María Dolores Tabernero, Angel Maíllo, Ana Espinosa, Ana Rasillo, Pedro Díaz, Juana Ciudad, Antonio López, Marta Merino, Jesús María Gonçalves, Angel Santos-Briz, Francisco Morales, Alberto Orfao The Journal of Molecular Diagnostics Volume 6, Issue 4, Pages 316-325 (November 2004) DOI: 10.1016/S1525-1578(10)60527-2 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Number of tumor cell clones present in meningioma tumors (n = 125) as detected by multicolor interphase FISH (iFISH) and flow cytometry analysis of cell DNA contents (FCM). The Journal of Molecular Diagnostics 2004 6, 316-325DOI: (10.1016/S1525-1578(10)60527-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Representative pictures of meningioma tumoral nuclei from a patient carrying two different tumor cell clones as defined by simultaneous hybridization for chromosomes 22 (green spots) and 9 (red spots): one clone shows monosomy 22 while the second clone displays two copies for chromosome 22 and four copies for chromosome 9. A corresponds to hybridizations performed on single cell suspensions while B shows hybridized nuclei from a tissue section from another area of the same tumor. The Journal of Molecular Diagnostics 2004 6, 316-325DOI: (10.1016/S1525-1578(10)60527-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Meningioma tumors (n = 125): correlation between the DNA index obtained by flow cytometry (FCM) and both the uncorrected (A) and corrected (B) chromosome indices obtained for each tumor cell clone according to the numerical chromosome abnormalities detected by interphase FISH analysis of 12 different chromosome probes. The Journal of Molecular Diagnostics 2004 6, 316-325DOI: (10.1016/S1525-1578(10)60527-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Meningioma tumors (n = 125): cytogenetic patterns found in the ancestral tumor cell clone identified, based on the analysis of 12 different chromosome-probes by multicolor interphase FISH. The Journal of Molecular Diagnostics 2004 6, 316-325DOI: (10.1016/S1525-1578(10)60527-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 5 Genetic heterogeneity of meningiomas: hypothetical intratumoral aneuploidization pathways for the 11 chromosomes (12 probes) analyzed which were recurrently observed (≥2% of the cases). Percentages show the incidence of cases with a tumor cell clone displaying a specific cytogenetic pattern. The Journal of Molecular Diagnostics 2004 6, 316-325DOI: (10.1016/S1525-1578(10)60527-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions