Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite–induced asthma Hee-Yeon Kim, MDa, Hae-Sim Park,

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Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite–induced asthma Hee-Yeon Kim, MDa, Hae-Sim Park, MDa, Yoon-Keun Kim, MDb, Jee-Woong Son, MDb, Hyun-Ah Kim, MSa, Jung-Hee Suh, MSa, Dong-Ho Nahm, MDa, Sang-Heon Cho, MDb, Kyung-Up Min, MDb, You-Young Kim, MDb Journal of Allergy and Clinical Immunology Volume 107, Issue 2, Pages 244-248 (February 2001) DOI: 10.1067/mai.2001.112123 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Protein staining of 12% SDS-PAGE of CRM extract. MW, Molecular weight. Journal of Allergy and Clinical Immunology 2001 107, 244-248DOI: (10.1067/mai.2001.112123) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 A, Binding pattern of IgE-binding components of CRM extract in 10 individual patients (lanes 1-10) , control sera (lane C) , and buffer (lane B) . M, Marker protein. B, Percentage of sera of CRM-allergic individuals showing specific IgE binding to the different proteins of CRM. Journal of Allergy and Clinical Immunology 2001 107, 244-248DOI: (10.1067/mai.2001.112123) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Two-dimensional PAGE analysis of CRM extract. A, Coomassie blue staining of 2-dimensional PAGE; B, IgE immunoblotting of 2-dimensional PAGE with the reactive pooled sera. Journal of Allergy and Clinical Immunology 2001 107, 244-248DOI: (10.1067/mai.2001.112123) Copyright © 2001 Mosby, Inc. Terms and Conditions