Blocking of Plexin B1 before rSema4A applications decreases the relative numbers of Treg cells and IL-2 secretion in human PBMCs. Human anti–Plexin B1.

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Blocking of Plexin B1 before rSema4A applications decreases the relative numbers of Treg cells and IL-2 secretion in human PBMCs. Human anti–Plexin B1 blocking Ab was added to separate wells 30 min before rSema4A or irrelevant protein additions. Blocking of Plexin B1 before rSema4A applications decreases the relative numbers of Treg cells and IL-2 secretion in human PBMCs. Human anti–Plexin B1 blocking Ab was added to separate wells 30 min before rSema4A or irrelevant protein additions. PBMCs were cultured with or without external rSema4A for 48 h. (A) The relative numbers of CD4+CD25highFoxp3+ cells were assessed in six flow cytometry settings for each cell culture condition, and the corresponding statistics were evaluated. (B) Cell culture supernatants from three separate experiments were assessed for the IL-2 content by sandwich ELISA. Note a positive correlation between an exogenous Sema4A dose and IL-2 production by PBMCs. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors