Volume 9, Issue 6, Pages (June 2002)

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Volume 9, Issue 6, Pages 721-729 (June 2002) Engineering Deoxysugar Biosynthetic Pathways from Antibiotic-Producing Microorganisms Leticia Rodrı́guez, Ignacio Aguirrezabalaga, Nerea Allende, Alfredo F Braña, Carmen Méndez, José A Salas Chemistry & Biology Volume 9, Issue 6, Pages 721-729 (June 2002) DOI: 10.1016/S1074-5521(02)00154-0

Figure 1 Map of pLN2 Only restriction sites flanking the different ole genes that were used for subcloning are indicated. Abbreviations are as follows: PermE*, erythromycin resistance promoter; tsr, thiostrepton resistance gene; bla, β-lactamase gene: pUC18, replication origin of pUC18; pIJ101, replication origin of pIJ101. Chemistry & Biology 2002 9, 721-729DOI: (10.1016/S1074-5521(02)00154-0)

Figure 2 Chemical Structure of 8-Demethyl-Tetracenomycin C and the Different Glycosylated Compounds Generated with the ElmGT Glycosyltransferase and pLN2 Derivatives Chemistry & Biology 2002 9, 721-729DOI: (10.1016/S1074-5521(02)00154-0)

Figure 3 HPLC Analysis of Cultures of Different Recombinant Strains Harboring pLN2 Derivatives and Producing 8DMTC Glycosylated Compounds (A) Strain harboring pLN2. (B) Strain harboring pLNR. (C) Strain harboring pLN2Δ. (D) Strain harboring pLNRHO. Chemistry & Biology 2002 9, 721-729DOI: (10.1016/S1074-5521(02)00154-0)

Figure 4 Enzymatic Steps Catalyzed by the Different 4-Ketoreductases Used in this Study Chemistry & Biology 2002 9, 721-729DOI: (10.1016/S1074-5521(02)00154-0)

Figure 5 Organization of ole Genes in pLN2 and Plasmid-Borne Constructs Derived from pLN2 Black triangles indicate the erythromycin resistance promoter. Red arrows indicate genes substituting ole genes in the different constructs. Abbreviations are as follows: Av, AvrII; Hp, HpaI; Nh, NheI; Pc, PacI; Sp, SpeI; Sh, SphI; Xb, XbaI. Chemistry & Biology 2002 9, 721-729DOI: (10.1016/S1074-5521(02)00154-0)

Figure 6 Proposed Pathways for the Biosynthesis of the Different NDP-Activated Deoxysugars Generated in this Work Chemistry & Biology 2002 9, 721-729DOI: (10.1016/S1074-5521(02)00154-0)