AbH AbF p=0.01 p=0.02 5 p=0.004 p=0.001 Fold increase in 4 NHS NHS+IgG NHS+AbH no serum HI NHS Fold increase in C3 deposition 1 2 3 4 5 p=0.02 p=0.001 AbH p=0.01 p=0.004 NHS+AbF AbF Supplementary Figure 2. Deposition of C3-related fragments on A549 lung cancer cells: Effect of autoantibodies F and H. Antibodies tested are IgG and human anti-CFH autoantibody from patients F and H (0.2 mg/ml), in the presence of normal human serum (NHS) as a source of complement or heat inactivated serum (HI-NHS). Fold increase in C3 deposition is reported relative to the baseline observed in the absence of serum.