Fig. 3. Wisper controls CF behavior and survival.

Slides:

Advertisements

Similar presentations

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

Advertisements

Fig. 7. Downstream effects of α-synuclein on mitochondria.

Fig. 2. LUM015 fluorescently labels tumor cells in mouse models of STS and breast cancer. LUM015 fluorescently labels tumor cells in mouse models of STS.

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

Transfer of miR-223 during neutrophil-epithelial cell interactions

NF1 silencing confers resistance of PC9 lung adenocarcinoma cells to erlotinib (erl). NF1 silencing confers resistance of PC9 lung adenocarcinoma cells.

A FOXO3a-BIM cascade mediates sensitivity to PARP and MEK inhibition

Fig. 6. C3 deficiency resulted in partial sparing of neuron loss in hippocampal CA3 in 16-month-old APP/PS1 mice. C3 deficiency resulted in partial sparing.

Fig. 2. Mechanism of PD-L1 down-regulation in NOD HSPCs.

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Fig. 3. LTB4 exhibits concentration-dependent effects on HLEC lymphangiogenesis and survival. LTB4 exhibits concentration-dependent effects on HLEC lymphangiogenesis.

Preceding knockdown of FoxO3 reduces inhibitory effect of UCN‐01 on human lung fibroblast proliferation and myofibroblast transdifferentiation in response.

Fig. 4. Biomechanical properties of treated tibiae.

Fig. 5. Wisper is associated with TIA1-related protein and regulates lysyl hydroxylase 2 expression. Wisper is associated with TIA1-related protein and.

Synergistic effect of S63845 with lapatinib, trastuzumab, or docetaxel

Fig. 2 TLR8 is aberrantly expressed on pDCs from SSc patients.

Fig. 3. A circadian rhythm in fibroblast wound-healing response.

Fig. 1. Identification of SE-associated lncRNAs.

Fig. 7. The PD-L1 defect is evident in HSPCs from T1D patients.

Fig. 2. GPC3 expression in normal and tumor tissues.

Expression of CD36 and psap in a TMA of human ovarian cancer patients

Fig. 5. Vascularization of human liver seed grafts.

Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture.

Fig. 4. Expression of HGF in liver ECs cooperates with NOX4 inhibition to enhance engraftment of regenerative hepatocytes. Expression of HGF in liver ECs.

Fig. 4. Actin polymerization rhythms are required for circadian regulation of adhesion and wound-healing efficacy by fibroblasts. Actin polymerization.

Persistence of CAR4 cells is reduced after sustained TCR engagement

Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 3. ERRα is involved in the regulation of OPLAH.

Fig. 1 BX795 suppresses HSV-1 infection.

Fig. 7 CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα. CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα.

Fig. 5. Nutlin-3 treatment rescues the proliferation and differentiation of NPCs in vitro. Nutlin-3 treatment rescues the proliferation and differentiation.

Fig. 7 DMN-Tre labeling of Mycobacterium tuberculosis is inhibited by tuberculosis drug cocktail, unlike auramine staining. DMN-Tre labeling of Mycobacterium.

Fig. 7. Genetic ablation of UCP2 compromised the protective effect of exogenous irisin on lung IR injury. Genetic ablation of UCP2 compromised the protective.

Fig. 3. TKI sensitivity assessed by the MANO method.

Loss of Keap1 contributes to LSCC pathogenesis by activating the NRF2–ROS pathway. Loss of Keap1 contributes to LSCC pathogenesis by activating the NRF2–ROS.

Fig. 1. Bexarotene promotes PPARδ activation to ameliorate the neurotoxicity of mutant huntingtin. Bexarotene promotes PPARδ activation to ameliorate the.

Fig. 3. HECTD2 contains a naturally occurring polymorphism at A19, which mislocalizes to the cytosol. HECTD2 contains a naturally occurring polymorphism.

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

Fig. 4. Acute lung injury in miR-223−/y mice.

Fig. 3 CSF1 is expressed in human melanoma.

Fig. 5 DMN-Tre labeling is selective for live mycobacteria.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Fig. 2. Cellular response to FolamiRs in vitro.

Fig. 1. Specificity of FolamiR uptake in cancer cells in culture.

Fig. 1 LB100 and LB102 specifically inhibit PP2A phosphatase activity and the growth of BCR-ABL+ cells. LB100 and LB102 specifically inhibit PP2A phosphatase.

CSF1 secretion by melanoma cells is induced by CTL-derived cytokines

Correlation of reovirus RNA/protein with proliferating tumor cells

Fig. 4 ALRN-6924 inhibits cellular proliferation and clonogenic capacity, and induces cell cycle arrest and apoptosis in AML cell lines. ALRN-6924 inhibits.

The effects of KY19382 on chondrocyte proliferation and differentiation. The effects of KY19382 on chondrocyte proliferation and differentiation. (A) ATDC5.

Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Effect of M-cadherin RNAi and GSK-3β inhibition on myogenic differentiation of C2C12 myoblasts. Effect of M-cadherin RNAi and GSK-3β inhibition on myogenic.

MK-8628 suppresses T lymphocyte proliferation.

Inhibition of Rho–ROCK–myosin switches from suppression to promotion of cell proliferation as matrix stiffness decreases. Inhibition of Rho–ROCK–myosin.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

Effect of FGF8 on R-C marker expression.

Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.

Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration,

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Fig. 2. LUM015 fluorescently labels tumor cells in mouse models of STS and breast cancer. LUM015 fluorescently labels tumor cells in mouse models of STS.

Fig. 4 Cdc42 enhances the cellular uptake of EVs.

CCG regulates cellular localization and blocks target gene expression of MRTF. A, SK-Mel-147 cells were cotransfected with RhoC and the SRE.L reporter.

Fig. 4. miR promotes cardiomyocyte proliferation through regulation of Hippo pathway kinases. miR promotes cardiomyocyte proliferation through.

miR can promote cardiomyocyte proliferation in the adult heart

Fig. 2 Increasing KLF17, CDH1, and LASS2 expression reduced malignant progression and promoted apoptosis of tumor cells. Increasing KLF17, CDH1, and LASS2.

Fig. 2. Mechanism of PD-L1 down-regulation in NOD HSPCs.

Fig. 3 Ect2 gene inactivation lowers cardiomyocyte endowment and is lethal in mice. Ect2 gene inactivation lowers cardiomyocyte endowment and is lethal.

PD and efficacy of AZD4785 in a KRAS wild-type lung cancer PDX model

Fig. 2. Cellular response to FolamiRs in vitro.

Presentation transcript:

Fig. 3. Wisper controls CF behavior and survival. Wisper controls CF behavior and survival. (A) Wisper expression in adult CFs after GapmeR transfection at increasing concentrations. Bars represent means normalized to control ± SEM (n ≥ 6). P values were determined by Student’s t test. (B and H) Gene expression measured by qRT-PCR in adult CFs (B) or lung fibroblasts (FBs) (H) after transfection with GapmeRs (10 nM, 48 hours; n ≥ 5) targeting Wisper (GM-Wisper) or scrambled GapmeRs (GM-Scr). P values were determined by two-way ANOVA (Fisher’s test). (C and I) Proliferation of adult CFs (C) or lung fibroblasts (I) after GapmeR transfection (10 nM) quantified by [H3]thymidine incorporation. Data are expressed as mean counts per million (cpm) ± SEM (n ≥ 3). P values were determined by two-way ANOVA (Bonferroni’s test). (D and J) Migration of adult CFs (D) or lung fibroblasts (J) after GapmeR transfection (10 nM) measured using a wound closure assay. Representative pictures are shown. Scale bars, 100 μM. Graphs show the percentage of wound closure at each time point ± SEM (n ≥ 3). P values were determined by two-way ANOVA (Bonferroni’s test). (E and K) Representative fluorescence-activated cell sorting (FACS) analysis of annexin V–positive adult CFs (E) or lung fibroblasts (K) after GapmeR transfection (10 nM). Graphs show the percentage of apoptotic cells ± SEM (n = 3). P values were determined by Student’s t test. (F) Bax expression–to–Bcl2 expression ratio in adult CFs after GM-Wisper or GM-Scr transfection (10 nM, 48 hours). Bars represent means ± SEM (n = 3). P values were determined by Student’s t test. (G) Wisper expression in adult CFs and lung fibroblasts after GM-Wisper transfection (10 nM). Bars represent means normalized to GM-Scr ± SEM (n ≥ 5). P values were determined by Student’s t test. Rudi Micheletti et al., Sci Transl Med 2017;9:eaai9118 Published by AAAS