HSPC and T‐cell transduction

Slides:

Advertisements

Similar presentations

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Advertisements

Impact of triple combination of gemcitabine‐based chemoirradiation with anti‐PD‐L1 in the KPC tumor allograft Impact of triple combination of gemcitabine‐based.

Statistical analysis of transgene expression in pigs generated by lentiviral gene transfer. Statistical analysis of transgene expression in pigs generated.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Expression analyses of KLK7 mRNA in the brains of AD patients

Loss of USP8 or HIF1α impairs endocytic recycling required for ciliogenesis Kinetic analysis of transferrin recycling measured by flow cytometry. Loss.

GLUT3 is a direct target of TCF4/β‐catenin

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

Spatial and temporal expression patterning of dysregulated genes from neurons in vivo Spatial and temporal expression patterning of dysregulated genes.

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

Impairment of Listeria protrusion formation by overexpression of the cytoplasmic tail of CD44. Impairment of Listeria protrusion formation by overexpression.

AAV8‐hAAT‐FGF21 treatment improves liver fibrosis

CAR T cell dose and percentages of CAR‐positive cells within CAR T cell products CAR T cell dose and percentages of CAR‐positive cells within CAR T cell.

Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent and cycling LGR5+ CRC cells Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent.

VAP‐A is recruited to interorganelle contact sites together with MOSPD2 VAP‐A is recruited to interorganelle contact sites together with MOSPD2 AHeLa cells.

Metabolic profile of Tfe3 KO mice

Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.

Preceding knockdown of FoxO3 reduces inhibitory effect of UCN‐01 on human lung fibroblast proliferation and myofibroblast transdifferentiation in response.

Phenogenetic networks of genes linked to Alzheimer's and Parkinson's disease reveal concordant phenotypes Phenogenetic networks of genes linked to Alzheimer's.

Clinical testing of calcineurin inhibitors cyclosporine A and tacrolimus in autoimmune disorders Clinical testing of calcineurin inhibitors cyclosporine.

Supraphysiological ARSA activity in LV.hARSA‐injected NHP

Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),

TFE3 prevents diet‐induced obesity and metabolic syndrome

[68Ga]Pentixafor PET/CT and [18F]FDG PET/CT A–DMaximum intensity projections (MIP) of [68Ga]Pentixafor (A) and [18F]FDG PET/CT (B) of a 68‐year‐old male.

Modules with causal links to ALS are associated with inflammation and enriched with TDP‐43 protein–protein interactions (PPIs)‏ Modules with causal links.

KLK7 is involved in the Aβ degradation activity

Expression of PD‐1 on PBMC and malignant exudate T cells

The upstream region of MUC5AC bound by SPDEF is required for the expression of MUC5AC in human lung cancer cells The upstream region of MUC5AC bound by.

Effect of VEGFA on ALDH+ population maintenance

Stromal fibromuscular AR regulates epithelium proliferation, ECM remodelling, neovasculature formation and immune cell infiltration in Pten deficient mice.

PD‐L1 silencing in antigen presenting DCs results in hyperactivated pro‐inflammatory TCRhigh CD8+ T cells. PD‐L1 silencing in antigen presenting DCs results.

T22‐GFP‐H6‐FdU prevents metastasis in the M5 patient‐derived model in a CXCR4‐dependent manner T22‐GFP‐H6‐FdU prevents metastasis in the M5 patient‐derived.

Stat3C/C mice are more prone to Th17 differentiation. A

WT‐HSCT, LV. IDS, and LV. IDS

DOK7 gene therapy enhances life span and motor activity in ALS mice

Characterization of the transcriptional responses in HSPC

IR induces cellular damage and a loss of nerves and SOX2 and SOX2+ progenitors can replenish mouse acinar cells in response to cholinergic mimetics IR.

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

Safety profile of reversibly immortalised human mesoangioblasts

Inhibition of NAMPT using the potent inhibitor FK866 sensitizes additional tumour cells to olaparib.A‐E.Sensitivity of the cell lines MDAMB468 (A), SUM149.

Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages during CVB3 infection Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages.

Feedback signalling through TP53‐CDKN1A‐GADD45A‐MAPK14‐GRB2‐TGFBRII‐TGFβ induces ROS production and maintains DDR. (A–C) MRC5 cells were transfected with.

Gene targeting of the therapeutic EGFP/PGK‐FANCA donor in the AAVS1 locus of HD CD34+ cells Gene targeting of the therapeutic EGFP/PGK‐FANCA donor in the.

LRRC25 inhibits RLR‐mediated type I IFN signaling pathway

Lack of RAD51 nuclear foci identifies PARPi‐sensitive PDX tumors

Scheme of FGF‐dependent regulation of TGFβ signaling in smooth muscle cells and endothelial cells Scheme of FGF‐dependent regulation of TGFβ signaling.

Quantification of phenotypes by genes and developmental stage

VCN and GFP distribution in LV.GFP‐injected NHPs

The antitumor activity of olaparib in PDXs identifies a subset of PARPi‐sensitive tumors The antitumor activity of olaparib in PDXs identifies a subset.

Generation and molecular characterization of LV producer cell lines

Drp1 knockdown prevents mitophagy

Rescue of motor neurons from death The ventral root of the fifth lumbar segment (L5). Rescue of motor neurons from death The ventral root of the fifth.

P2X4R blockade increases autoimmune inflammation

Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R cells to PORCN inhibition Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R.

Etifoxine modulation of T‐cell activity during EAE

T22‐GFP‐H6‐FdU‐induced depletion of CXCR4‐overexpressing cancer cells in tumor tissue T22‐GFP‐H6‐FdU‐induced depletion of CXCR4‐overexpressing cancer cells.

Dual‐recombinase fluorescence lineage tracing of EMT in KPF (FSF‐KrasG12D/+;Trp53frt/+;Pdx1‐Flp);αSMA‐Cre;R26Dual mice Dual‐recombinase fluorescence lineage.

The MEK inhibitor CI‐1040 reverses thickening, fibrosis, and angiogenesis in the parietal peritoneum of Cav1−/− mice upon exposure to PD fluidWT or Cav1−/−

Favorable safety profile of LV‐mediated intracerebral gene therapy

Sox2 is essential for SLG regeneration following radiation injury

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

POLG defects cause degeneration of enhancer‐active regions of CNS

P2X4R blockade increases pro‐inflammatory gene expression after EAE

Single‐molecule dynamics of the active mutant, hDIA1(R1204X), in XTC cells Single‐molecule dynamics of the active mutant, hDIA1(R1204X), in XTC cells A–DGFP‐tagged.

CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair depigmentation CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair.

CAR T‐cell generation in human PBMC‐transplanted mice

SEMA3C regulates prostate cancer cell growth

Niacin suppresses pro‐inflammatory cytokine expression in macrophages in DSS‐induced colitis in mice Niacin suppresses pro‐inflammatory cytokine expression.

Presentation transcript:

HSPC and T‐cell transduction HSPC and T‐cell transduction AGating strategy for HSPC and CFC population analysis. FMO: fluorescence minus one; arrows indicate sub‐gating from the previously gated population.B, CMean with SEM (MOI 10 and 100, n = 4) or range (MOI 300, n = 2) of the CFC composition in (B) erythroid progenitors (CD235a‐positive cells) and (C) myeloid progenitors (CD33‐positive cells), 2 weeks after transduction with LV produced by transient transfection (white bars) or by LV‐GFP producer cell line (black bars), expressed as fold change to untreated cells (UNT).DGating strategy for T‐cell population analysis.E, FMean with SEM (“cell line”, n = 4) or range (“transfection”, n = 2) of the population composition in CD4‐positive (E) or CD8‐positive (F) cells, 1 week after LV transduction, expressed as fold change to untreated cells (UNT). Michela Milani et al. EMBO Mol Med. 2017;emmm.201708148 © as stated in the article, figure or figure legend