HDAC Inhibitors Finally Open Up: Chromatin Accessibility Signatures of CTCL  Christopher J. Ott, Catherine J. Wu  Cancer Cell  Volume 32, Issue 1, Pages 1-3 (July 2017) DOI: 10.1016/j.ccell.2017.06.008 Copyright © 2017 Elsevier Inc. Terms and Conditions

Figure 1 Use of ATAC-seq to Reveal Chromatin Accessibility Profiles of CTCL (A) Flow-sorted populations of CD4+ T cells from healthy donors and CTCL patients used for ATAC-seq profiling. Leukemic cell populations were purified from host T cells via the unique Vβ receptor. (B) ATAC-seq on each population revealed profiles of active cis-regulatory elements, with evidence of population-distinct gene signatures, unique transcription factor circuits associated with response or resistance to HDACi, and correlations with subsets of memory T cells. (C) Chromatin states during HDACi therapy were assessed, revealing that response is associated with pharmacodynamic increase in baseline regulatory element accessibility and apparent normalization of host T cell chromatin signatures. Cancer Cell 2017 32, 1-3DOI: (10.1016/j.ccell.2017.06.008) Copyright © 2017 Elsevier Inc. Terms and Conditions