Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation Magnús Gottfredsson, Helga.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Laboratory diagnosis and biosafety issues of biological warfare agents

In-vitro interaction of azithromycin and fluoroquinolones against gram-positive and gram-negative bacteria Matthew W. Ruble, Deborah H. Gilbert, Stephen.

Gut bacterial microbiota and obesity

M.A.C. Broeren, Y. Maas, E. Retera, N.L.A. Arents

Approach to diagnosis of infective endocarditis

A new method for normalized interpretation of antimicrobial resistance from disk test results for comparative purposes G. Kronvall, G. Kahlmeter, E.

Margrét Valdimarsdóttir, Helga Erlendsdóttir, Sigurdur Gudmundsson

Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China Z. Xu, L. Shi, C. Zhang, L. Zhang, X. Li,

The efficacy and safety of daptomycin: first in a new class of antibiotics for Gram- positive bacteria M.J. Rybak Clinical Microbiology and Infection

Herpes zoster in non-hospitalized children

Antimicrobial Activity of SCH 27899, Oligosaccharide Member of the Everninomycin Class with a Wide Gram-Positive Spectrum Ronald N. Jones, M.D., Mary.

In vitro activity of cefpirome and vancomycin in combination against gentamicin- susceptible and gentamicin-resistant Staphylococcus aureus A. Carricajo,

A new method for normalized interpretation of antimicrobial resistance from disk test results for comparative purposes G. Kronvall, G. Kahlmeter, E.

Gentamicin susceptibility in Escherichia coli related to the genetic background: problems with breakpoints L. Jakobsen, D. Sandvang, V.F. Jensen, A.M.

Hydatid disease Clinical Microbiology and Infection

Antimicrobial drug use and infection control practices associated with the prevalence of methicillin-resistant Staphylococcus aureus in European hospitals

How to evaluate and predict the ecologic impact of antibiotics: the pharmaceutical industry view from research and development R. Bax Clinical Microbiology.

Revision of linezolid disk diffusion quality control guidelines for testing Staphylococcus aureus ATCC 25923: an independent seven-laboratory trial D.J.

W. Witte, B. Pasemann, C. Cuny Clinical Microbiology and Infection

How to evaluate and predict the epidemiologic impact of antibiotic use in humans: the pharmacoepidemiologic approach D. Guillemot Clinical Microbiology.

In vitro activity, postantibiotic effect and human monocyte activity of grepafloxacin against Legionella species Jacques Dubois, Claude St-Pierre Clinical.

Structure of grepafloxacin relative to activity and safety profile

Pharmacodynamics of doxycycline

Inga Odenholt, Elisabeth Löwdin, Otto Cars

Fourth-generation cephalosporins: a review of in vitro activity, pharmacokinetics, pharmacodynamics and clinical utility Javier Garau, Walter Wilson,

C-3′Quaternary Ammonium Cephems, a New Wave of Cephalosporins

Training for the infectious diseases speciality in Norway

Pharmacodynamic properties of HMR 3004, a novel ketolide, on respiratory pathogens, enterococci and Bacteroides fragilis demonstrated by studies of time.

Low prevalence of methicillin-resistant Staphylococcus aureus with reduced susceptibility to glycopeptides in Belgian hospitals C. Nonhoff, O. Denis,

Epidemiology of opportunistic infections in AIDS patients

D. Esel, B. Sümerkan, S. Kocagöz Clinical Microbiology and Infection

Laboratory diagnosis and biosafety issues of biological warfare agents

Bacterial resistance—the clinical challenge

Comparative in vitro activity of ertapenem against bacterial pathogens isolated from patients with lower respiratory tract infections P.S. Hicks, B.

Comparative in vitro pharmacodynamics of BO-2727, meropenem and imipenem against Gram-positive and Gram-negative bacteria Inga Odenholt, Elisabeth Löwdin,

Sepsis and neutropenia induced by clozapine

Uncommon opportunistic fungi: new nosocomial threats

G. Kronvall Clinical Microbiology and Infection

In vitro selection of antibiotic resistance in Pseudomonas aeruginosa

Evolving epidemiology of invasive Haemophilus infections in the post-vaccination era: results from a long-term population-based study M.R. Berndsen,

Laboratory diagnosis of Clostridium difficile disease

Pathogenesis of catheter-related infections: lessons for new designs

Antibacterial drug discovery in the 21st century

Community-acquired pneumonia: epidemiologic and clinical consideration

Pandemic lineages of extraintestinal pathogenic Escherichia coli

P.M. Hawkey Clinical Microbiology and Infection

Comparative bactericidal activities of daptomycin, glycopeptides, linezolid and tigecycline against blood isolates of Gram-positive bacteria in Taiwan

Lack of antimicrobial activity of sodium heparin for treating experimental catheter-related infection due to Staphylococcus aureus using the antibiotic-lock.

Fibronectin concentrations in catheter sepsis

Andrew M. Middleton, Maureen V. Chadwick, Harold Gaya

M.B. Kerrn, N. Frimodt-Møller, F. Espersen

P. Kaltsas, S. Want, J. Cohen Clinical Microbiology and Infection

European guidelines for diagnosis and management of patients with suspected herpes simplex encephalitis Miles Denton, Kevin G Kerr, Fraser Lewis Clinical.

Clinical Microbiology and Infection

Overview of cefixime use in community-acquired infections

Sanford Chodosh Clinical Microbiology and Infection

Facilitating learning and change in physicians: Implications for a system of continuing medical education in Europe Robert D. Fox Clinical Microbiology.

Endotoxin release from Escherichia coli after exposure to tobramycin: dose-dependency and reduction in cefuroxime-induced endotoxin release J. SjöUlin,

Abstracts Clinical Microbiology and Infection

New oral cephalosporins in pediatric community-acquired infections

Comparison of PCR-based methods for typing Escherichia coli

In vitro activity of a new echinocandin, LY303366, and comparison with fluconazole, flucytosine and amphotericin B against Candida species† C.B. Moore,

B.A. Cunha Clinical Microbiology and Infection

G.C. Schito Clinical Microbiology and Infection

Bactericidal activity and synergy studies of BAL9141, a novel pyrrolidinone-3- ylidenemethyl cephem, tested against streptococci, enterococci and methicillin-resistant.

The first glycopeptide-intermediate Staphylococcus aureus in Oman

Impact of antibiotic restrictions: the patient's perspective

The future of diagnostic bacteriology

Vincent Calvez, Marc Grandadam Clinical Microbiology and Infection

Presentation transcript:

Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation Magnús Gottfredsson, Helga Erlendsdóttir, Adalsteinn Gudmundsson, Sigurdur Gudmundsson Clinical Microbiology and Infection Volume 2, Issue 2, Pages 99-104 (October 1996) DOI: 10.1111/j.1469-0691.1996.tb00213.x Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Examples of growth (CFU/mL) and [3H] thymidine incorporation (counts/min) curves of E. coli ATCC 25922. Circles, unexposed control organisms; triangles, organisms during PAE after exposure to doxycyclin 4 x MIC for 1 h. Closed symbols, viability counts (log10(CFU/mL)); open symbols, [3H] thymidine incorporation (counts/min). In the example, the PAE determined by viable counts was 1.2 h and by [3H] thymidine incorporation 1.6 h. Clinical Microbiology and Infection 1996 2, 99-104DOI: (10.1111/j.1469-0691.1996.tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Comparison of the [3H] thymidine incorporation method and viability counting for PAE determination in S. aureus. Bacterial counts post-drug removal were > 104 CFU/mL (range: 2.5 × 104 to 6 × 105 CFU/mL). Columns represent means from two to three experiments. Bars represent range. Di, dicloxacillin; Ge, gentamicin; Va, vancomycin; Rif, rifampin. a: data from one experiment. Clinical Microbiology and Infection 1996 2, 99-104DOI: (10.1111/j.1469-0691.1996.tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Comparison of the [3H]thymidine incorporation method and viability counting for PAE determination in E. coli. Bacterial counts post-drug removal were >104 CFU/mL (range: 1.5 × 104 to 2.3 × 106 CFU/mL). Columns represent means from two to three experiments. Bars represent range. Ge, gentamicin; To, tobramycin; Dx, doxycycline; Rif, rifampin; Im, imipenem. Clinical Microbiology and Infection 1996 2, 99-104DOI: (10.1111/j.1469-0691.1996.tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 4 Correlation between CERT in S. aureus and E. coli as determined by viability counting and [3H] thymidine incorporation. Closed circles: E. coli; open circles: S. aureus. Clinical Microbiology and Infection 1996 2, 99-104DOI: (10.1111/j.1469-0691.1996.tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions