Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather.

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Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather S. Wolff, M.D., Jolene R. Fredrickson, M.S., David L. Walker, M.S., Gaurang S. Daftary, M.D., Dean E. Morbeck, Ph.D. Fertility and Sterility Volume 99, Issue 3, Pages 847-854.e2 (March 2013) DOI: 10.1016/j.fertnstert.2012.10.046 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Blastocyst rates of one-cell mouse embryos from different mouse embryos to increasing concentrations of cumene peroxide in mineral oil. *P<.05; **P<.01 vs. control. (B) LC50 values for cumene hydroperoxide in mineral oil for blastocyst development of one-cell mouse embryos from different strains of mice. Fertility and Sterility 2013 99, 847-854.e2DOI: (10.1016/j.fertnstert.2012.10.046) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Number of cells per blastocyst for embryos of different strains treated with cumene hydroperoxide in mineral oil. *P<.05 vs. control. Fertility and Sterility 2013 99, 847-854.e2DOI: (10.1016/j.fertnstert.2012.10.046) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Blastocyst rates of one-cell mouse embryos from different mouse embryos to increasing concentrations of Triton X-100 in culture media. *P<.05; **P<.01 vs. control. (B) LC50 values for Triton X-100 in culture media for blastocyst development of one-cell mouse embryos from different strains of mice. Fertility and Sterility 2013 99, 847-854.e2DOI: (10.1016/j.fertnstert.2012.10.046) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Number of cells per blastocyst for embryos of different strains treated with Triton X-100 in culture media. *P<.05 vs. control. Fertility and Sterility 2013 99, 847-854.e2DOI: (10.1016/j.fertnstert.2012.10.046) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 (A) Blastocyst rates of one-cell mouse embryos from different mouse embryos to increasing osmolality. *P<.05; **P<.01 vs. control. (B) LC50 values for osmolality of culture media for blastocyst development of one-cell mouse embryos from different strains of mice. Fertility and Sterility 2013 99, 847-854.e2DOI: (10.1016/j.fertnstert.2012.10.046) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Number of cells per blastocyst for embryos of different strains treated with increasing osmolality. *P<.05 vs. control. Fertility and Sterility 2013 99, 847-854.e2DOI: (10.1016/j.fertnstert.2012.10.046) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions