What type of gene transfer requires a bacteriophage?

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Presentation transcript:

What type of gene transfer requires a bacteriophage?

What type of gene transfer requires a bacteriophage? Transduction

What is a bacteriophage?

What is a bacteriophage? It is a virus that infects bacteria

What type of phage is necessary for specialized transduction?

What type of phage is necessary for specialized transduction? It requires a lysogenic phage A lysogenic phage integrates its DNA into the bacterial chromosome and becomes a prophage

When does specialized transduction occur?

When does specialized transduction occur? Only when the prophage incorrectly excises itself from the bacterial chromosome and enters into a lytic cycle Prophage Lytic cycle

When does transformation occur?

When does transformation occur? When a cell is competent and picks up DNA that has been released into the environment

What is generalized transduction?

What is generalized transduction? When a lytic phage accidentally transfers a random piece of chromosomal DNA

What type of mutation is shown here?

This is a missense mutation.

What type of mutation is shown here?

This is a nonsense mutation.

2-aminopurine is an example of __________?

2-aminopurine is an example of a nucleoside analog It is a chemical mutagen

How does Non-ionizing radiation act as a mutagen?

How does Non-ionizing radiation act as a mutagen? It causes the formation of thymine-dimers

What enzymes are needed for “dark repair” of thymine dimers?

What enzymes are needed for “dark repair” of thymine dimers? A nuclease cuts on either side of the dimer DNA polymerase replaces the missing strand DNA ligase seals the sugar-phosphate backbone