Tadhg Ó Cróinín, Marguerite Clyne, Brendan Drumm Gastroenterology

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Molecular mimicry of ferret gastric epithelial blood group antigen A by Helicobacter mustelae Tadhg Ó Cróinín, Marguerite Clyne, Brendan Drumm Gastroenterology Volume 114, Issue 4, Pages 690-696 (April 1998) DOI: 10.1016/S0016-5085(98)70582-7 Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 Analysis of gastric epithelial cells and H. mustelae strain 12198 using whole-cell antiserum raised in rabbits against H. mustelae strain 12198 and preimmune serum. Lanes 1 and 7, H. mustelae strain 12198; lanes 2 and 8, ferret gastric epithelial cells; lane 3, ferret duodenal epithelial cells; lane 4, ferret colonic epithelial cells; lane 5, rabbit gastric epithelial cells; and lane 6, human gastric epithelial cells. Lanes 1–6 were incubated with H. mustelae antiserum, and lanes 7 and 8 were incubated with preimmune serum. Molecular masses are indicated (in kilodaltons) on the left. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 2 Flow-cytometric analysis of ferret gastric cells stained with (A) H. mustelae antiserum, (B) preimmune serum, and (C) H. mustelae antiserum absorbed with ferret and rabbit epithelial cells. The mean fluorescence intensity is indicated in the top right of each histogram. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 3 Detection of cross-reacting bands using whole cell antiserum raised against H. mustelae strain 12198 adsorbed with H. mustelae whole cells or rabbit and ferret epithelial cells. Lanes 1 and 2, H. mustelae strain 12198; and lanes 3–5, ferret gastric epithelial cells. Lanes 1 and 3 were incubated with H. mustelae antiserum, lanes 2 and 4 were incubated with H. mustelae antiserum adsorbed with rabbit and ferret epithelial cells, and lane 5 was incubated with H. mustelae antiserum adsorbed with H. mustelae strain 12198. Molecular masses are indicated (in kilodaltons) on the left. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 4 Identification of blood group antigens on H. mustelae, H. pylori, and ferret gastric cells. Lanes 1, 4, and 7, ferret gastric cells; lanes 2, 5, and 8, H. mustelae strain 12198; and lanes 3, 6, and 9, H. pylori strain PU3. Lanes 1–3 were incubated with monoclonal antibodies against blood group antigen A, lanes 4–6 were incubated with monoclonal antibodies against Lewis x, and lanes 7–9 were incubated with monoclonal antibodies against Lewis y. The molecular masses (in kilodaltons) are indicated on the left. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 5 Flow-cytometric analysis of ferret gastric cells stained with (A) monoclonal antibodies raised against blood group antigen A and (B) normal mouse immunoglobulins. The mean fluorescence intensity is indicated in the top right of each histogram. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 6 (A) Treatment of ferret gastric cells with proteinase K and sodium metaperiodate. Lane 1, ferret gastric cells; lane 2, ferret gastric cells incubated with sodium metaperiodate; and lane 3, ferret gastric cells incubated with proteinase K. (B) Treatment of H. mustelae with proteinase K and sodium metaperiodate. Lane 1, H. mustelae strain 12198; lane 2, H. mustelae 12198 treated with sodium metaperiodate; and lane 3, H. mustelae 12198 treated with proteinase K. All lanes were incubated with anti–H. mustelae serum. Molecular masses (in kilodaltons) are indicated on the left. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 7 Cross-reactivity of various antibodies with ferret gastric epithelial cells. (A) Ferret gastric tissue stained with preimmune serum from a rabbit. (B) Ferret gastric tissue stained with H. mustelae antiserum raised in a rabbit. (C) Ferret gastric tissue stained with monoclonal antibodies against human blood group A. (D) Ferret gastric epithelia stained with normal mouse immunoglobulin G. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 7 Cross-reactivity of various antibodies with ferret gastric epithelial cells. (A) Ferret gastric tissue stained with preimmune serum from a rabbit. (B) Ferret gastric tissue stained with H. mustelae antiserum raised in a rabbit. (C) Ferret gastric tissue stained with monoclonal antibodies against human blood group A. (D) Ferret gastric epithelia stained with normal mouse immunoglobulin G. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 7 Cross-reactivity of various antibodies with ferret gastric epithelial cells. (A) Ferret gastric tissue stained with preimmune serum from a rabbit. (B) Ferret gastric tissue stained with H. mustelae antiserum raised in a rabbit. (C) Ferret gastric tissue stained with monoclonal antibodies against human blood group A. (D) Ferret gastric epithelia stained with normal mouse immunoglobulin G. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 7 Cross-reactivity of various antibodies with ferret gastric epithelial cells. (A) Ferret gastric tissue stained with preimmune serum from a rabbit. (B) Ferret gastric tissue stained with H. mustelae antiserum raised in a rabbit. (C) Ferret gastric tissue stained with monoclonal antibodies against human blood group A. (D) Ferret gastric epithelia stained with normal mouse immunoglobulin G. Gastroenterology 1998 114, 690-696DOI: (10.1016/S0016-5085(98)70582-7) Copyright © 1998 American Gastroenterological Association Terms and Conditions