Mean and difference Raman spectra of T cells isolated from spleen of sham mice and endotoxemic mice for time points 1, 4, 10, and 30 d. Mean and difference.

Slides:

Advertisements

Similar presentations

Clonal bias of CD56−CD16+ NK cell subpopulations.

Advertisements

Volume 21, Issue 11, Pages (December 2017)

B-cell precursors in the spleen of Eμ-Myc mice are reduced by mTORC1 inhibition. B-cell precursors in the spleen of Eμ-Myc mice are reduced by mTORC1 inhibition.

Resveratrol modulates DSS-induced neutrophils during colitis.

Attema et al., Supplementary Figure 1

MR1-TM inhibits the development of severe ATD and pSS in CD28−/− NOD

by Daniel L. Barber, Katrin D. Mayer-Barber, Lis R. V

MP cells, but not pathogen-elicited effector CD4+ T lymphocytes, rapidly produce IFN-γ during T. gondii infection independently of pathogen antigens. MP.

Depletion of CD169+ cells leads to increased expression of CD25 on enterotoxin A–specific Vβ3+ T cells. Depletion of CD169+ cells leads to increased expression.

PD-1 deficiency promotes the development of splenic GCs in male mice, and MR1 dissolves GCs in both genders. PD-1 deficiency promotes the development of.

Volume 3, Issue 5, Pages (November 2014)

Viral load and CD4+ T cells in CD8+ T cell–depleted YU-2–infected MISTRG mice. Viral load and CD4+ T cells in CD8+ T cell–depleted YU-2–infected MISTRG.

Mean and difference Raman spectra of spleen tissue from endotoxemic mice and sham mice and PCA-LDA. Mean and difference Raman spectra of spleen tissue.

rSema4A potentiates Treg cells in CD4+ T cell cultures.

TIGIT expression in naive T cells is accelerated by coculture with si-tolerant T cells at an early-stimulation stage. TIGIT expression in naive T cells.

Fluorescence-stained images and respective bright-field images of mouse spleen tissue sections area investigated using Raman spectroscopy. Fluorescence-stained.

Smaller T cell zone FRC areas in aged spleens.

Functions of myeloid cells.

False-color Raman images of CD4+T cells and CD8+ T cells from control and LPS-treated animals after day 1, 4, 10, and 30 obtained by k-means cluster analysis.

rSema4A increases the relative number of CD4+CD25+ HuT cells.

rSema4A has no direct effect on Treg cells in the in vitro cultures.

Specific depletion of TFH and LCMV-specific CD4 T cells.

CCL21 localizes to T cell zone FRCs in young and aged spleens.

CXCR5+/+ TFH cells are dispensable for maintenance of the LCMV-specific antibody response. CXCR5+/+ TFH cells are dispensable for maintenance of the LCMV-specific.

Specific depletion of CD4-DTR–derived CD4 T cells.

Volume 13, Issue 6, Pages (November 2015)

Volume 21, Issue 11, Pages (December 2017)

CD160−/− mice have impaired clearance of oral L

Immune cell populations, activation, and NKG2D and H60a expression in the spleen of untreated and antibiotic-treated Klrk1+/+ and Klrk1−/− NOD mice. Immune.

Loss of DGKζ and Cbl-b results in a greater percentage of splenic CD8+ T cells with an activated phenotype. Loss of DGKζ and Cbl-b results in a greater.

Extracellular mitochondria cause neutrophil recruitment.

Volume 15, Issue 3, Pages (April 2016)

Volume 11, Issue 3, Pages (September 2012)

Cytosolic entry of Lm required for CD8α+ DC recruitment.

PCA-LDA of Raman spectra of T cells isolated from spleen of sham mice and endotoxemic mice for time points 1, 4, 10 and 30 d. PCA-LDA of Raman spectra.

False-color Raman images of spleen tissue sections from control and LPS-treated animals obtained by k-means cluster analysis. False-color Raman images.

IL-27 contributes to IR expression by lung T cells during toxoplasmosis. IL-27 contributes to IR expression by lung T cells during toxoplasmosis. (A) Il27p28-GFP.

Differential expression of TRM markers by donor- and recipient-derived T cells with time. Differential expression of TRM markers by donor- and recipient-derived.

The mucosal environment regulates CD160 expression on IELs

Comparison of male and female CD8+ T cells primed with different amounts of cognate Ag. (A) Schematic of experimental design: 1 × 104 gBT-I CD8+ T cells.

IFN-γ–producing cells in the spleen and lungs postimmunization.

Analysis of peripheral blood CD4+CD25+ T-cells after B6

TSG-6 suppressed APC activation in vitro and in vivo.

IRF4 expression regulates the sensitivity of CD8+ T cells to IL-2, IL-15, and IL-7. IRF4 expression regulates the sensitivity of CD8+ T cells to IL-2,

CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria.

MP cells, but not pathogen-elicited effector CD4+ T lymphocytes, rapidly produce IFN-γ during T. gondii infection independently of pathogen antigens. MP.

CD8α+, CD8α−, and MoDCs from NOD.hCD205 mice express hCD205.

NOX1-deficient mice do not respond to GKT771 and exhibit reduced B16-F10 tumor growth associated with decreased production of angiogenic factors and minor.

PD1 targeting alters the recruitment of immune cells to MC38 CRC tumors. PD1 targeting alters the recruitment of immune cells to MC38 CRC tumors. MC38.

Slc7a5 expression in preosteoclasts is reduced in ovariectomized mice.

MK-8628 modulates transcriptional signatures of activated T lymphocytes. MK-8628 modulates transcriptional signatures of activated T lymphocytes. Gene.

MK-8628 suppresses T lymphocyte proliferation.

Pie charts (for all the mice together) and bar graph (for individual mouse) showing relative percentage of T cells and B cells present in the Raman spectroscopy–investigated.

Phenotypic characterization of splenic and islet-infiltrating B-cells in 12-week-old NOD female mice. Phenotypic characterization of splenic and islet-infiltrating.

LG NK cells appear to be conventional in phenotype.

Transgenic c-Maf mediates upregulation of type 2 cytokines in NOD CD4, but not CD8, cells. Transgenic c-Maf mediates upregulation of type 2 cytokines in.

CypD-deficient mice are susceptible to Mtb infection.

Overexpression of IL-27 upregulates expression of multiple IR by T cells in vivo. Overexpression of IL-27 upregulates expression of multiple IR by T cells.

Conversion of CD11bhighCD27high NK cells into MDSCs leads to CD11bhighCD27high and CD11bhighCD27low NK cell reduction. Conversion of CD11bhighCD27high.

Possible mechanisms of recognition of MHCIlow tumor cells by NSCs

BLITC reporter for monitoring of GvHD.

Representative macrophage staining images only from the knee joints of CAIA mice injected s.c. with GalNAc–MASP-3–siRNA duplex 2 or GalNAc–luciferase–siRNA.

Dysregulated NF-κB activation in Il1r8+/+/lpr and Il1r8−/−/lpr mice.

Mice with a B cell–specific deletion of Ets1 have increased memory phenotype B cells but no increase in switching to IgG1. Mice with a B cell–specific.

The effect of TGFβ on the post-RT increase of Tregs in tumors.

LSECtin, expressed by B16 cells, inhibits the tumor-specific immune responses both in vivo and in vitro. LSECtin, expressed by B16 cells, inhibits the.

miR-146a is highly expressed selectively on γδ27− T cells.

Mice with a B cell–specific deletion of Ets1 do not have increased CD4+ T cell activation. Mice with a B cell–specific deletion of Ets1 do not have increased.

Volume 25, Issue 4, Pages (October 2006)

E2 induces IL-17–producing γδ+ T cells in the FGT

Presentation transcript:

Mean and difference Raman spectra of T cells isolated from spleen of sham mice and endotoxemic mice for time points 1, 4, 10, and 30 d. Mean and difference Raman spectra of T cells isolated from spleen of sham mice and endotoxemic mice for time points 1, 4, 10, and 30 d. Top row: mean Raman spectra of (A) CD4+CD8+ T cells, (B) CD4+ T cells, and (C) CD8+ T cells isolated from spleen of sham C57BL/6 mice and LPS-insulted C57BL/6 mice (time point of LPS insult is shown on right axis). Bottom row: difference Raman spectra between (D) CD4+CD8+ T cells, (E) CD4+ T cells, and (F) CD8+ T cells from sham mice (spectra in black) and LPS-insulted mice (spectra in red). Negative peaks belong to LPS-treated cells, and positive peaks belong to healthy cells. The spectra are shifted in y-axis for clarity. For each time point, pooled spleen obtained from 9 to 12 mice (both male and female) per condition have been used (see Supplemental Table IA). A minimum of three biological repetition and Raman spectroscopic investigations were done for each time point (see Supplemental Table IA). T cells were isolated, and CD4+ T cells and CD8+ T cells were purified using automated negative magnetic-assisted cell sorting using standard isolation kit. Anuradha Ramoji et al. ImmunoHorizons 2019;3:45-60 Copyright © 2019 The Authors