Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for.

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Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation detection T. Prammananan, W. Cheunoy, D. Taechamahapun, J. Yorsangsukkamol, S. Phunpruch, P. Phdarat, M. Leechawengwong, A. Chaiprasert Clinical Microbiology and Infection Volume 14, Issue 5, Pages 446-453 (May 2008) DOI: 10.1111/j.1469-0691.2008.01951.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Schematic view of the rpoB gene fragments targeted by the multiplex allele-specific (MAS)-PCR. Short arrows depict the primers, and long double-headed arrows represent the PCR products obtained with the Mycobacterium tuberculosis complex-specific primers (196 bp) or the allele-specific primers (176, 162, 129 or 115 bp, respectively); figure not to scale. Clinical Microbiology and Infection 2008 14, 446-453DOI: (10.1111/j.1469-0691.2008.01951.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Examples of the results obtained using the multiplex allele-specific (MAS)-PCR method. Lanes: M, 25-bp DNA ladder; 1–4, Mycobacterium tuberculosis isolates with a mutation at codon 531; 5, rifampicin-susceptible isolate; 6 and 7, isolates with a mutation at codon 526; 8 and 9, isolates with a mutation at codon 516; 10, isolate with a double mutation at codons 511 and 516; 11, isolate with a deletion between codons 513 and 516; 12–14, rifampicin-susceptible isolates; 15, 1 ng of purified M. tuberculosis H37Rv DNA (positive control); 16, positive internal marker generated by mixing the amplified products of each primer pair. Clinical Microbiology and Infection 2008 14, 446-453DOI: (10.1111/j.1469-0691.2008.01951.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions