Surface Engineered Bacteria Engineered to Bind and Signal

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Cell membranes are composed of two phospholipid layers.

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Presentation transcript:

Surface Engineered Bacteria Engineered to Bind and Signal Fusion Protein OmpA – C terminal insertion OmpA-Loop1 insertion AIDA-1 – N terminal insertion FecA – loop insertion Membrane Protein Add a slide about the concept of the library. 1

Selecting/enriching for surface engineered bacteria Direct Selection Direct magnetic beads Indirect selection MACS FACS

Various Cell Sorting Experiments with his and strep2 - Indirect Bead Assays Various Cell Sorting Experiments with his and strep2 - Assay Results Direct Bead Assays

After Separation Test: Cell Sorting with AIDA1 + sender constructs (with his and strep2) - Assay Results Before Separation strep2 his

Results: Cell Selection Assays are a Success! Selecting for surface engineered bacteria (such as his and strep2) is possible through either direct or indirect cell separation.