A) B) C) Derivative reporter (-Rn) Temperature (°C) Temperature (°C)

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A) B) C) Derivative reporter (-Rn) Temperature (°C) Temperature (°C) 65 70 75 80 85 90 95 65 70 75 80 85 90 95 65 70 75 80 85 90 95 Temperature (°C) Temperature (°C) Temperature (°C) Supplementary Figure 1. Dissociation curves following qPCR analysis of mcrA gene in rumen DNA samples from faunated and defaunated wethers. Example of sample from the solid phase of faunated (F) and medium-term defaunated (D-) wethers (A), sample from the solid phase of long-term defaunated wethers (D+) (B), and sample from the liquid phase of D+ wethers (C).