rSema4A increases the relative number of CD4+CD25+ HuT cells.

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rSema4A increases the relative number of CD4+CD25+ HuT cells. rSema4A increases the relative number of CD4+CD25+ HuT cells. Cells were cultured in vitro in cRPMI with or without rSema4A for 48 h. Cells were analyzed for CD25 expression (A and B). Live HuT cell dot plots (A) and histograms (B) show the levels of CD25 marker expression. (B) Marker positivity on HuT cells in cultures with rSema4A is shown in black line with transparent background histograms or solid black color histogram (for 100 ng and 1 μg of rSema4A, correspondingly), whereas CD25 expression on cells cultured with medium alone is shown in solid gray color. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors