Correlation of quinolone resistance levels and differences in basal and quinolone- induced expression from three qnrA-containing plasmids J.M. Rodríguez-Martínez,

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Presentation transcript:

Correlation of quinolone resistance levels and differences in basal and quinolone- induced expression from three qnrA-containing plasmids J.M. Rodríguez-Martínez, C. Velasco, A. Pascual, I. García, L. Martínez-Martínez Clinical Microbiology and Infection Volume 12, Issue 5, Pages 440-445 (May 2006) DOI: 10.1111/j.1469-0691.2006.01389.x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Southern hybridisation of plasmid DNA digested with EcoRI and probed with qnrA. Lanes: 1 and 9, λ-HindIII DNA size marker; 2, negative control Escherichia coli J53AzR; 3, 5 and 7, clinical isolates UAB1, N5, 1960; 4, 6 and 8 correspond to UAB1, N5 and 1960-derived transconjugants. Clinical Microbiology and Infection 2006 12, 440-445DOI: (10.1111/j.1469-0691.2006.01389.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Quantitative determination of the plasmid copy number of the qnrA gene. Dot-blot analysis was performed following serial dilution (300 ng, 150 ng, 75 ng and 37.5 ng) of plasmid DNA (pDNA). Lanes: 1, 2 and 3, UAB1, N5 and 1960-derived transconjugants; 4, negative control Escherichia coli J53AzR; 5, 6 and 7, clinical isolates UAB1, N5 and 1960. Clinical Microbiology and Infection 2006 12, 440-445DOI: (10.1111/j.1469-0691.2006.01389.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Comparative analysis of the increase of qnrA expression in the presence of ciprofloxacin (CIP) and moxifloxacin (MXF). (A) Basal expression of the qnrA gene in the three different transconjugants. (B) qnrA transcription in the presence of CIP and MXF at 0.4 × MIC for 4 h in transconjugants (TC) derived from UAB1, N5 and 1960 isolates. NI, cells cultured in the absence of antibiotics. Clinical Microbiology and Infection 2006 12, 440-445DOI: (10.1111/j.1469-0691.2006.01389.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions