New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies Francisco Dominguez, Ph.D., Marcos Meseguer,

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Presentation transcript:

New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies Francisco Dominguez, Ph.D., Marcos Meseguer, Ph.D., Belen Aparicio-Ruiz, Ph.D., Paloma Piqueras, Ph.D., Alicia Quiñonero, Ph.D., Carlos Simón, M.D. Fertility and Sterility Volume 104, Issue 4, Pages 908-914 (October 2015) DOI: 10.1016/j.fertnstert.2015.06.032 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Graphic presentation of the relationships between the presence of (A) interleukin (IL) 6, (B) stem cell factor (SCF), and (C) interferon (IFN) α2 and the percentages of known implanted embryos. Fertility and Sterility 2015 104, 908-914DOI: (10.1016/j.fertnstert.2015.06.032) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Percentage of implanted embryos whose cell division times were inside or outside the optimal ranges defined by Meseguer et al. (13). The four graphs show the ranges and implantation status for: (A) cc2: the second cell cycle duration (i.e., the time from division to a 2-cell blastomere embryo to division to a 3-cell blastomere embryo); (B) t5: division to 5 cells; (C) s2: synchrony of divisions from the 2-cell to the 4-cell stage; and (D) tEB: the time required for expansion of the blastocele cavity. Fertility and Sterility 2015 104, 908-914DOI: (10.1016/j.fertnstert.2015.06.032) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Hierarchic classification of embryos based on first the presence of Il-6 in the spent culture media and second duration of second cell cycle, cc2, i.e., the time from division to a 2-blastomere until division to a 3-blastomere embryo. This classification generates four embryo categories with increasing expected implantation potential (right to left). The implantation rates in relation to the embryo categories defined in the hierarchic classification-tree model are presented below each category. Fertility and Sterility 2015 104, 908-914DOI: (10.1016/j.fertnstert.2015.06.032) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions