Production of recombinant KRBV NS1 and antiserum reactivity.

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Presentation transcript:

Production of recombinant KRBV NS1 and antiserum reactivity. Production of recombinant KRBV NS1 and antiserum reactivity. SDS-PAGE and Western blot analysis of unboiled and unreduced untransfected COS-7L cell lysate (lanes 1); KRBV NS1-transfected COS-7L cell lysate (lanes 2), and concentrated supernatant (lanes 3); HisTrap column-purified KRBV NS1 (lanes 4) show NS1 monomers at approximately 50 kDa and oligomers at 100 and 150 kDa and higher molecular masses. Lanes L contain the molecular weight marker Precision Plus Protein Kaleidoscope (Bio-Rad). Panel A, with anti-V5 epitope MAb (lanes rearranged for figure clarity); panel B1, with immunized mouse serum (mouse 2); panel B2, with negative mouse serum. (C) IFA with anti-KRBV NS1 mouse serum of COS-7L cells (mock transfected and transfected with KRBV NS1) and C6/36 cells infected with cISFs (mouse 2 antiserum). Agathe M. G. Colmant et al. mSphere 2017; doi:10.1128/mSphere.00262-17