Does supplementation of in-vitro culture medium with melatonin improve IVF outcome in PCOS? Mi Kyoung Kim, Eun A. Park, Hyung Joon Kim, Won Yun Choi,

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Presentation transcript:

Does supplementation of in-vitro culture medium with melatonin improve IVF outcome in PCOS? Mi Kyoung Kim, Eun A. Park, Hyung Joon Kim, Won Yun Choi, Jung Hyun Cho, Woo Sik Lee, Kwang Yul Cha, You Shin Kim, Dong Ryul Lee, Tae Ki Yoon Reproductive BioMedicine Online Volume 26, Issue 1, Pages 22-29 (January 2013) DOI: 10.1016/j.rbmo.2012.10.007 Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Melatonin concentrations in culture media measured by ELISA. (A) Media were collected from granulosa cell (GC) culture at day 1 (24h), day 2 (48h) and day 3 (72h) after human chorionic gonadotrophin treatment. (B) Media of cultures with/without five cumulus-oocyte-complexes (COC) were collected before and after in-vitro maturation (IVM). Data are mean±SEM. All experiments were replicated three times. Different lower-case letters indicate a significant difference (P<0.05). Reproductive BioMedicine Online 2013 26, 22-29DOI: (10.1016/j.rbmo.2012.10.007) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Quantification of aralkylamine acetylserotonin O-methyltransferase (ASMT; A and C) and N-acetyltransferase (AANAT; B and D) mRNA expression in cultured granulosa cells (GC) with (C and D) and without (A and B) 50μg/ml tryptophan supplementation. Cultured GC were collected at 0, 24, 48 and 72h after HCG treatment, total RNA was extracted and real-time reverse-transcription PCR analysis assessed gene expression. Data are mean±SEM. All experiments were replicated 10 times. Different lower-case letters indicate a significant difference (P<0.05). Reproductive BioMedicine Online 2013 26, 22-29DOI: (10.1016/j.rbmo.2012.10.007) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Effect of melatonin on the clinical outcomes from IVM IVF-embryo transfer using non-stimulation (A) and human chorionic gonadotrophin priming (B) in patients with polycystic ovary syndrome. MEL+=IVM medium with 10μmol/l melatonin; MEL−=IVM medium without 10μmol/l melatonin (control). Asterisks indicate significant differences (P<0.05). Reproductive BioMedicine Online 2013 26, 22-29DOI: (10.1016/j.rbmo.2012.10.007) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions