Quantitation OF ARGS aggrecan fragments in synovial fluid, serum and urine from osteoarthritis patients F.M. Germaschewski, C.J. Matheny, J. Larkin,

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Presentation transcript:

Quantitation OF ARGS aggrecan fragments in synovial fluid, serum and urine from osteoarthritis patients F.M. Germaschewski, C.J. Matheny, J. Larkin, F. Liu, L.R. Thomas, J.S. Saunders, K. Sully, C. Whittall, Y. Boyle, G. Peters, N.M. Graham Osteoarthritis and Cartilage Volume 22, Issue 5, Pages 690-697 (May 2014) DOI: 10.1016/j.joca.2014.02.930 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Comparison of serum ARGS neoepitope concentrations (ng/mL) in healthy (n = 20), OA (n = 20) and OA-TKR (n = 20). OA subject data is the mean value from all individuals from all visits. Shaded box represents median and 25%/75% percentile, whiskers represent the min/max of ARGS neoepitope values across the 20 subjects in each group, circles represent mean values. Results show a significant difference between OA-TKR and healthy (***P = 0.0002) and between OA-TKR and OA (**P = 0.01). Osteoarthritis and Cartilage 2014 22, 690-697DOI: (10.1016/j.joca.2014.02.930) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 (a) Analysis of mean diurnal (hours) and inter-day variation on visits 1–3 (V1–3) in ARGS neoepitope concentrations levels (ng/mL) from duplicate serum samples from individual OA patients (n = 20 subjects). (b) Serum ARGS neoepitope concentrations presented as box and whiskers plot. Shaded box represents median and 25%/75% percentile, whiskers represent the min/max of ARGS neoepitope values across the 20 subjects in each group, circles represents mean values. Results show no significant diurnal or inter-day variability either in mean serum ARGS neoepitope concentrations or intra subject concentrations over time (P > 0.05). (c) Analysis of mean diurnal (hours) and inter-day variation on visits 1–3 (V1–3) in ARGS neoepitope concentrations levels (ng/mmol) from duplicate creatinine corrected urine samples from individual OA patients (n = 20 subjects). (d) Creatinine corrected urine ARGS neoepitope concentrations presented as box and whiskers plot. Shaded box represents median and 25%/75% percentile, whiskers represent the min/max of ARGS neoepitope values across the 20 subjects in each group, circles represents mean values. Results show no significant diurnal or inter-day variability either in mean creatinine corrected urine ARGS neoepitope concentrations or intra subject concentrations over time (P > 0.05). Osteoarthritis and Cartilage 2014 22, 690-697DOI: (10.1016/j.joca.2014.02.930) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Correlation plot between serum (mean ng/mL from two duplicate samples from each donor) and synovial ARGS neoepitope shows no significant linear correlation (P = 0.50, r = 0.17). The linear fit and 95% confidence interval are shown in the plot. Matched serum and SF from OA-TKR subjects with measurable SF (n = 18). Osteoarthritis and Cartilage 2014 22, 690-697DOI: (10.1016/j.joca.2014.02.930) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Correlation between serum or synovial ARGS neoepitope or creatinine corrected ARGS neoepitope (mean ng/mL from two duplicate samples) vs subject age, number of concurrent OA joints and combined WOMAC score (pain, stiffness, physical). Data presented from age and sex-matched OA serum and urine (blue O) (n = 20) and OA-TKR matched serum and urine (red +) (n = 20) and OA-TKR subjects with measurable SF (n = 18). Data was Log10 transformed and shows correlation of serum ARGS neoepitope vs age in both OA and OA-TKR groups (P = 0.03, P = 0.01 respectively). A significant correlation of creatinine corrected ARGS with age was observed which was strongest in the OA-TKR subjects (r = 0.56, P = 0.01). Mild correlations were observed between SF ARGS and the number of concurrent OA joints (P = 0.14) and total WOMAC (P = 0.12). Regression analysis with Pearson's correlation coefficient. Osteoarthritis and Cartilage 2014 22, 690-697DOI: (10.1016/j.joca.2014.02.930) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions