Alessandro Fasciani, M. D. , Guido Bocci, M. D. , Jing Xu, M. D. , Ph

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Presentation transcript:

Three-dimensional in vitro culture of endometrial explants mimics the early stages of endometriosis Alessandro Fasciani, M.D., Guido Bocci, M.D., Jing Xu, M.D., Ph.D., Ryszard Bielecki, D.V.M., Ellen Greenblatt, M.D., Nicholas Leyland, M.D., Robert F Casper, M.D. Fertility and Sterility Volume 80, Issue 5, Pages 1137-1143 (November 2003) DOI: 10.1016/S0015-0282(03)02164-2

FIGURE 1 Microscopic pictures representing tissue outgrowth from the endometrial explant within the three-dimensional fibrin matrix after (A) 7, (B) 15, (C) 60, and (D) 90 days of culture. Bar = 500 μm. Fasciani. In vitro 3-dimensional model of endometriosis. Fertil Steril 2003. Fertility and Sterility 2003 80, 1137-1143DOI: (10.1016/S0015-0282(03)02164-2)

FIGURE 2 (A) Phase contrast photomicrograph of unfixed endometrial stromal cells in vitro forming multilayered structures with a central cavity. (B–D) Photomicrographs of paraffin-fixed sections of endometrial outgrowths in three-dimensional fibrin matrix showing typical histological architecture of endometrial cells, (B) typical endometrial glands staining with anticytokeratin, (C) glands and stroma viewed under phase-contrast, and (D) normal nuclear morphology with the DAPI fluorescent stain. Bar = 25 μm. Fasciani. In vitro 3-dimensional model of endometriosis. Fertil Steril 2003. Fertility and Sterility 2003 80, 1137-1143DOI: (10.1016/S0015-0282(03)02164-2)

FIGURE 3 (A) Three-dimensional tube formation of endothelial cells into the fibrin matrix at day 35 of culture (bar = 10 μm). (B) Microscopic phase-contrast pictures. (C) Hematoxylin-eosin stain of capillaries sprouting from the endometrial outgrowth at the seventh week of culture (bar = 250 μm). Fasciani. In vitro 3-dimensional model of endometriosis. Fertil Steril 2003. Fertility and Sterility 2003 80, 1137-1143DOI: (10.1016/S0015-0282(03)02164-2)

FIGURE 4 Positive immunohistochemical reaction for (A) CD31 and (B) vimentin antigens in endothelial cells growing into fibrin gel matrix. (C) Negative control: omission of primary antibody results in capillaries without immunoreactivity (bar = 25 μm). Fasciani. In vitro 3-dimensional model of endometriosis. Fertil Steril 2003. Fertility and Sterility 2003 80, 1137-1143DOI: (10.1016/S0015-0282(03)02164-2)