Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells Amélie Bourdiec, Syed-Furquan Ahmad,

Slides:

Advertisements

Similar presentations

J. Ben-Nagi, J. Miell, D. Mavrelos, J. Naftalin, C. Lee, D. Jurkovic

Advertisements

Fig. 2. Effect of PGE2 on VEGF production in NPDFs as determined by RT-PCR (A, C) and ELISA (B, D). (A, B) RT-PCR and ELISA showed that the relative ratio.

Red Wine Prevents the Acute Negative Vascular Effects of Smoking

Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia Go Kuwahara,

Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages B.P. Deng, Y. Zhang, Q.J. Wang, X.F. Xu, H. Zhang,

Differential expression of vascular endothelial growth factor-A isoforms in the mouse uterus during early pregnancy Lisa M. Walter, Peter A.W. Rogers,

Volume 78, Issue 3, Pages (August 2010)

Zhao Jing, M. D. , Zhang Qiong, M. D. , Wang Yonggang, M. D

Expression of KRAS in the endometrium of early pregnant mice and its effect during embryo implantation Xia Long, Min Zhang, Xuemei Chen, Junlin He, Yubin.

Volume 68, Issue 5, Pages (November 2005)

Heparin and low-molecular-weight heparins modulate the decidualization of human endometrial stromal cells Herbert Fluhr, M.D., Julia Spratte, B.S., Jens.

Differential regulation of Akt phosphorylation in endometriosis

S. S. Pullamsetti, R. Savai, W. Janssen, B. K. Dahal, W. Seeger, F

L. Aghajanova, L.C. Giudice Reproductive BioMedicine Online

Expression of vascular endothelial growth factor-A and its receptor-1 in a luteal endometrium in patients with repeated in vitro fertilization failure

P27 and its ubiquitin ligase Skp2 expression in endometrium of IVF patients with repeated hormonal stimulation Shirly Lahav-Baratz, Mara Koifman, Edmond.

Delphine Haouzi, Hervé Dechaud, Said Assou, John De Vos, Samir Hamamah

Thyroid-stimulating hormone receptor and thyroid hormone receptors are involved in human endometrial physiology Lusine Aghajanova, M.D., Ph.D., Anneli.

Oral and topical boswellic acid attenuates mouse osteoarthritis

Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans Qian Feng,

The science of implantation emerges blinking into the light

Withdrawal of GnRH agonist decreases oestradiol and VEGF concentrations in high responders Li-Jun Ding, Bin Wang, Xiao-Yue Shen, Gui-Jun Yan, Ning-Yuan.

Nuclear factor–kappaB: a main regulator of inflammation and cell survival in endometriosis pathophysiology Reinaldo González-Ramos, M.D., Ph.D., Sylvie.

Volume 69, Issue 8, Pages (April 2006)

MiR-483–5p and miR-486–5p are down-regulated in cumulus cells of metaphase II oocytes from women with polycystic ovary syndrome Lin Shi, Shan Liu, Wanqiu.

Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis Xiaochen Qi, Yanxin.

Tracey A. Edgell, Luk J.F. Rombauts, Lois A. Salamonsen

Patrick Kibangou Bondza, Ph. D. , Christine N. Metz, Ph. D

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Reprint of: Uterine plasticity and reproductive fitness

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell– derived factor 1 in human endometrial stromal cells Hidetaka.

The human oviduct transcriptome reveals an anti-inflammatory, anti-angiogenic, secretory and matrix-stable environment during embryo transit A.P. Hess,

Differential expression of vascular endothelial growth factor angiogenic factors in different endometrial compartments in women who have an elevated progesterone.

Volume 67, Issue 4, Pages (April 2005)

Oestrogen and progesterone action on endometrium: a translational approach to understanding endometrial receptivity Steven L. Young Reproductive BioMedicine.

Side population cells contribute to the genesis of human endometrium

Tumor necrosis factor α up-regulates endometrial milk fat globule–epidermal growth factor 8 protein production via nuclear factor κB activation, resulting.

Sphingosine–sphingosine-1-phosphate pathway regulates trophoblast differentiation and syncytialization Ambika T. Singh, Arunasalam Dharmarajan, Irving.

Apoptosis in normal ovaries of women with and without endometriosis

A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation Stacey C. Schutte,

Leiomyoma-derived transforming growth factor-β impairs bone morphogenetic protein-2- mediated endometrial receptivity Leo F. Doherty, M.D., Hugh S. Taylor,

Role of GnRH–GnRH receptor signaling at the maternal-fetal interface

Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in.

Electroacupuncture decreases the progression of ovarian hyperstimulation syndrome in a rat model Li Chen, Hai-Xiang Sun, You-Bing Xia, Liu-Cai Sui, Ji.

L. Simon, I. Proutski, M. Stevenson, D. Jennings, J. McManus, D

Abnormally Differentiating Keratinocytes in the Epidermis of Systemic Sclerosis Patients Show Enhanced Secretion of CCN2 and S100A9 Joanna Nikitorowicz-Buniak,

Pro-endometriotic niche in endometriosis

Metformin modulates IL-8, IL-1β, ICAM and IGFBP-1 expression in human endometrial stromal cells Ariane Germeyer, Julia Jauckus, Markus Zorn, Bettina.

Abnormally Differentiating Keratinocytes in the Epidermis of Systemic Sclerosis Patients Show Enhanced Secretion of CCN2 and S100A9 Joanna Nikitorowicz-Buniak,

Modulation of Collagen Metabolism by the Topical Application of Dehydroepiandrosterone to Human Skin Mi Hee Shin, Gi-eun Rhie, Chi-Hyun Park, Kyu Han.

J. Ben-Nagi, J. Miell, D. Mavrelos, J. Naftalin, C. Lee, D. Jurkovic

IL-6 Secreted from Cancer-Associated Fibroblasts Mediates Chemoresistance in NSCLC by Increasing Epithelial-Mesenchymal Transition Signaling Yasushi.

Ambika T. Singh, Jeffrey A. Keelan, Frank Sieg

Decreased expression of FOXA2 promotes eutopic endometrial cell proliferation and migration in patients with endometriosis Anping Lin, Juan Yin, Chao.

Up-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic.

Prokineticin 1, homeobox A10, and progesterone receptor messenger ribonucleic acid expression in primary cultures of endometrial stromal cells isolated.

Murat Ulukus, M. D. , E. Cagnur Ulukus, M. D. , Ege N

Intra-cycle variation of the uterine cavity indentation assessed with three-dimensional ultrasound in natural and stimulated cycles Sotirios H. Saravelos,

Lusine Aghajanova, M. D. , Ph. D. , Signe Altmäe, M. Sc

Requirement of heart and neural crest derivatives–expressed transcript 2 during decidualization of human endometrial stromal cells in vitro Hisayuu Shindoh,

Macrophage migration inhibitory factor expression in the intrauterine endometrium of women with endometriosis varies with disease stage, infertility status,

Philip Kressin, M. D. , Eva-Maria Wolber, Ph. D. , Harald Wodrich, Ph

A.K.I. Kuroki, Masayuki Iyoda, Takanori Shibata, Tetsuzo Sugisaki

Toward gene therapy of endometriosis: adenovirus-mediated delivery of dominant negative estrogen receptor genes inhibits cell proliferation, reduces cytokine.

Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis Tetsuya Hirata, M.D., Ph.D., Yutaka.

Local injury of the endometrium induces an inflammatory response that promotes successful implantation Yulia Gnainsky, Ph.D., Irit Granot, Ph.D., Paulomi.

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Microvascular density, vascular endothelial growth factor A, and its receptors in endometrial blood vessels in patients with menorrhagia Miriam Mints,

Differences in gene expression in the proliferative human endometrium

Estrogen and selective estrogen receptor modulators regulate vascular endothelial growth factor and soluble vascular endothelial growth factor receptor.

Presentation transcript:

Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells Amélie Bourdiec, Syed-Furquan Ahmad, Asmaa Lachhab, Ali Akoum Reproductive BioMedicine Online Volume 32, Issue 1, Pages 85-95 (January 2016) DOI: 10.1016/j.rbmo.2015.09.011 Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Effect of decidualization on endometrial stromal cell receptivity and decidual marker expression. (A) Secretion of prolactin was measured by enzyme-linked immunosorbent assay in the culture supernatants of the decidualized and non-decidualized (NDC) endometrial stromal cells. (B, C and D), mRNA expression levels of LIFR, HOXA10, and HOXA11, respectively, were assessed by quantitative real-time polymerase chain reaction and normalized using glyceraldehyde-3-phosphate dehydrogenase. Student's t-test was used to compare the decidualized and control non-decidualized endometrial stromal cells (n = 5). Values are means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001. DC = decidualized; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; LIFR = leukaemia inhibitory factor receptor; NDC = non-decidualized; PRL = prolactin. Reproductive BioMedicine Online 2016 32, 85-95DOI: (10.1016/j.rbmo.2015.09.011) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Effect of endometrial stromal cell decidualization on the expression of IL1 system members. mRNA expression levels of IL1R1, IL1R2 and IL1RN were assessed using quantitative real-time polymerase chain reaction and normalized using glyceraldehyde-3-phosphate dehydrogenase. IL1Rs, IL1RN and IL1B protein expression levels for the membrane-bound and intracytoplasmic forms in the total protein extracts and the soluble or secreted forms in the culture supernatants were determined by enzyme-linked immunosorbent assay. Student's t-test was used to the compare decidualized and control nondecidualized ESC cultures (n = 5). Values are means ± SEM, *P < 0.05; ***P < 0.001. DC = decidualized; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; NDC = non-decidualized. Reproductive BioMedicine Online 2016 32, 85-95DOI: (10.1016/j.rbmo.2015.09.011) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Effect of endometrial stromal cell decidualization on IL1 family members IL18 and IL33. mRNA expression levels of IL18R1 and ST2 were assessed by quantitative real-time polymerase chain reaction and normalized using glyceraldehyde-3-phosphate dehydrogenase. IL18Rs, ST2, IL18 and IL33 protein expression levels for the membrane-bound forms in total protein extracts and the soluble or secreted forms in the culture supernatants for were determined by enzyme-linked immunosorbent assay. Student's t-test was used to compare the decidualized and control non-decidualized endometrial stromal cell cultures (n = 5). Values are means ± SEM; *P < 0.05; **P < 0.01. DC = decidualized; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; NDC = non-decidualized. Reproductive BioMedicine Online 2016 32, 85-95DOI: (10.1016/j.rbmo.2015.09.011) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 IL33 localization in proliferative and mid-secretory phase human endometrium. Representative photomicrographs showing immunohistochemical staining of IL33 in mid-secretory (A, B) and proliferative (C, D) phase human endometrium. Photomicrographs are representative of 5 tissues/group. Scale bars = 50 µm. Reproductive BioMedicine Online 2016 32, 85-95DOI: (10.1016/j.rbmo.2015.09.011) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 Effect of endometrial stromal cell (ESC) decidualization on the secretion of angiogenic factors. Secretion of VEGF, TIMP3, MMP9, RANTES, MCP1 and MIF (A, B, C, D, E and F, respectively) was assessed in the culture supernatants of the decidualized or non-decidualized ESC using an enzyme-linked immunosorbent assay. Student's t-test was used to compare the decidualized and non-decidualized ESC (n = 5). Values are means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; compared with non-decidualized ESC cultures using Student's t-test. DC = decidualized; MCP-1 = monocyte chemoattractant protein-1; MIF = migration inhibitory factor; MMP = matrix metallopeptidase; NDS = non-decidualized; RANTES = regulated on activation, normal T cell expressed and secreted; TIMP-3 = metallopeptidase inhibitor 3; VEGF = vascular endothelial cell growth factor. Reproductive BioMedicine Online 2016 32, 85-95DOI: (10.1016/j.rbmo.2015.09.011) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions