Volume 101, Issue 11, Pages (December 2011)

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Volume 101, Issue 11, Pages 2843-2853 (December 2011) Measuring Kinetic Dissociation/Association Constants Between Lactococcus lactis Bacteria and Mucins Using Living Cell Probes Doan Thanh Lam Le, Yann Guérardel, Pascal Loubière, Muriel Mercier-Bonin, Etienne Dague Biophysical Journal Volume 101, Issue 11, Pages 2843-2853 (December 2011) DOI: 10.1016/j.bpj.2011.10.034 Copyright © 2011 Biophysical Society Terms and Conditions

Figure 1 Representative SEM and epifluorescence images of the biologically functionalized AFM probe, for L. lactis cells immobilized onto AFM tip and cantilever. (A) Si3N4 AFM probe. (B) AFM probe after preadsorption of PEI. (C and D) Lacto-probe of MG1820 and IBB477, respectively. (E) Bright-field microscopy image of the MG1820 lacto-probe (total bacteria). (F) Epifluorescence image of the MG1820 lacto-probe after CFDA staining. Biophysical Journal 2011 101, 2843-2853DOI: (10.1016/j.bpj.2011.10.034) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 2 AFM height (A) and deflection (C) images of the PGM layer formed on polystyrene. (B) Scratch test on a cross section. Biophysical Journal 2011 101, 2843-2853DOI: (10.1016/j.bpj.2011.10.034) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 3 (A and B) Adhesion maps. (C and D) Histograms representing the adhesion forces. (E and F) Typical force-distance curves presented for (A, C, and E) the MG1820 lacto-probe after contact with the PGM-coated surface, and (B, D, and F) the IBB477 lacto-probe after contact with the PGM-coated surface. Biophysical Journal 2011 101, 2843-2853DOI: (10.1016/j.bpj.2011.10.034) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 4 (A) Schematic representation of the AFM blocking test with free PGM or S-PGM. (B and C) Histograms of interaction forces before and after incubation of the lacto-probe with S-PGM and PGM solutions for (B) MG1820 and (C) IBB477. Biophysical Journal 2011 101, 2843-2853DOI: (10.1016/j.bpj.2011.10.034) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 5 Loading-rate dependence of specific (A) and nonspecific (B) interaction forces between L. lactis and PGM, for IBB477. Biophysical Journal 2011 101, 2843-2853DOI: (10.1016/j.bpj.2011.10.034) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 6 Contact-time dependence of the adhesion probability for MG1820 (♦) and IBB477 (▪). Biophysical Journal 2011 101, 2843-2853DOI: (10.1016/j.bpj.2011.10.034) Copyright © 2011 Biophysical Society Terms and Conditions