Organotypic Skin Culture

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Research Techniques Made Simple: Organotypic Skin Culture Ji Won Oh 1,2, Tsai-Ching Hsi 1, Christian Fernando Guerrero-Juarez 1, Raul Ramos 1, Maksim V.

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Organotypic Skin Culture Ji Won Oh, Tsai-Ching Hsi, Christian Fernando Guerrero-Juarez, Raul Ramos, Maksim V. Plikus Journal of Investigative Dermatology Volume 133, Issue 11, Pages 1-4 (November 2013) DOI: 10.1038/jid.2013.387 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Reconstituted epidermal organotypic culture systems (OCSs). The OCSs, reconstituted with keratinocytes and fibroblasts derived from a human donor with psoriasis (far right), displayed high expression of tumor necrosis factor α (TNF-α) and IFN-γ cytokines in epidermis (pink areas, arrows), similar to psoriasis skin (arrows). IL-6 remains high in both normal and psoriasis OCSs. Reprinted with permission from Barker et al. (2004). Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.387) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Flowchart of an organotypic epidermal culture system. Adapted from Kalabis et al. (2012). Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.387) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Flowchart of in vitro hair follicle culture. Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.387) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 The effect of IL-6 in humans hair follicle culture. (a) Addition of recombinant human IL-6 into human hair follicle cultures significantly inhibits the hair shaft elongation rate as compared with control. (b–f) The number of proliferating cells in the matrix of cultured human hair follicles, as measured by immunostaining for Ki-67 marker, significantly decreased in the presence of IL-6 (b). Also compare Ki-67 expression in (d) (IL-6 treated) versus (c) (control). Reprinted with permission from Kwack et al. (2012). Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.387) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Journal of Investigative Dermatology 2013 133, 1-4DOI: (10. 1038/jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Journal of Investigative Dermatology 2013 133, 1-4DOI: (10. 1038/jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions