Capture of CK+ and CK− complex aneuploid CTCs in breast, ovarian, or colorectal cancer. Capture of CK+ and CK− complex aneuploid CTCs in breast, ovarian,

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Capture of CK+ and CK− complex aneuploid CTCs in breast, ovarian, or colorectal cancer. Capture of CK+ and CK− complex aneuploid CTCs in breast, ovarian, or colorectal cancer. A, representative images illustrating detection of HER2+/CK+ and HER2+/CK− CTCs. Both cells display a > 2.2 HER2/centromere 17 ratio, confirming positive HER2 amplification. B, comparison of total CK+/CD45−, CK+/CD45−/HER2+ and CK−/CD45−/HER2+ cells from advanced-stage breast cancer patients. C, capture of circulating ovarian (top) and colorectal (bottom) carcinoma cells that stain for CK. Subsequent FISH shows an ovarian cancer cell with trisomy in chromosome 8 (blue, arrows) and monosomy in region 20Q11 (red, arrows), whereas the colorectal cancer cell has trisomy in chromosome 8 and tetrasomy in chromosome 17 (orange, arrows). D, capture of CK− circulating ovarian (top) and colorectal (bottom) carcinoma cells. FISH of an ovarian cancer cell with trisomy in chromosome 8 (blue), monosomy in chromosome 11 (green), and tetrasomy in region 20Q11 (orange), whereas the colorectal cancer cell has trisomy in chromosomes 8 (blue) and 11 (green) and monosomy in chromosome 17. The average number of total CK, complex aneuploid CK+, and CK− circulating tumor cells per milliliter of blood is shown for (E) ovarian and (F) colorectal cancer patients. *P < 0.05; **P = 0.007. Chad V. Pecot et al. Cancer Discovery 2011;1:580-586 ©2011 by American Association for Cancer Research