LATS2 is a tumor suppressor in a mouse lumB breast cancer model.

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LATS2 is a tumor suppressor in a mouse lumB breast cancer model. LATS2 is a tumor suppressor in a mouse lumB breast cancer model. (A) Relative total tumor weight, as percentage of total body weight of 3-mo-old Lats2-CKO PyMT and WT-PyMT littermate controls (n = number of mice); mean ± SEM; * P-value < 0.05. (B) Three mammary glands (see the Materials and Methods section) from Lats2-CKO PyMT and WT-PyMT littermate control mice were histologically scored. The most advanced pathological lesion from each mouse was tallied. (C) Representative H&E-stained sections from WT-PyMT (adenoma/MIN) and Lats2-CKO PyMT (carcinoma); scale bar = 500 μm. (D) Expression levels of Lats2 mRNA in WT-PyMT tumors of different histological stages, analyzed by RT-qPCR; mean ± SEM. (E) Left panel: Heatmap representing hierarchical clustering of global expression patterns of tumors from Lats2-CKO PyMT (n = 4) and WT-PyMT littermate controls (n = 3). Each tumor was taken from a different mouse. Standardized rld values are shown for differentially expressed genes (P-value < 0.05, n = 1131); ad = adenoma/MIN, car = carcinoma. Right panel: PCA of the most differentially expressed genes between Lats2-CKO PyMT and WT-PyMT tumors (adjP-value < 0.05), deduced from RNA-seq analysis. (F) GSEA of 1,131 genes ranked by fold change (red to blue gradient) between WT-PyMT and Lats2-CKO PyMT tumors (P-value < 0.05) and compared with genes down-regulated in LATS2L (versus LATSH) human lumB tumors (vertical black lines). Noa Furth et al. LSA 2018;1:e201800171 © 2018 Furth et al.