Alex Westerband, MD, Joseph L. Mills, MD, John M. Marek, MD, Ronald L

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Presentation transcript:

Immunocytochemical determination of cell type and proliferation rate in human vein graft stenoses Alex Westerband, MD, Joseph L. Mills, MD, John M. Marek, MD, Ronald L. Heimark, PhD, Glenn C. Hunter, MD, Stuart K. Williams, PhD Journal of Vascular Surgery Volume 25, Issue 1, Pages 64-73 (January 1997) DOI: 10.1016/S0741-5214(97)70322-7 Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 A, Duplex scan–derived peak systolic graft flow velocity and markedly elevated diastolic velocity obtained at site of a vein graft stenotic lesion. Also note wide spectral broadening. B, Arteriogram shows corresponding focal distal juxtaanastomotic lesion. Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 A, Duplex scan–derived peak systolic graft flow velocity and markedly elevated diastolic velocity obtained at site of a vein graft stenotic lesion. Also note wide spectral broadening. B, Arteriogram shows corresponding focal distal juxtaanastomotic lesion. Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Morphologic assessment of human vein graft stenoses by hematoxylin and eosin (H&E), Masson's trichrome stain; single-label immunocytochemistry with anti-PCNA, double immunolabeling for PCNA and different cell-specific markers. A, Hypercellularity noted on H&E stain with severe luminal narrowing (original magnification, 3.125 ×). B, Abundant extracellular matrix, high collagen content (blue-green), as seen with Masson's trichrome stain (original magnification, 12.5 ×). C, Immunocytochemical labeling for PCNA. PCNA-positive nuclei appear as dark red and are present in cells adjacent to the residual lumen in this specimen (original magnification, 31 ×). D, Double immunolabeling for PCNA and α-smooth muscle actin. Here PCNA-positive nuclei appear as dark black (peroxidase reaction, shown with arrowhead) and α-smooth muscle actin–positive cytoplasms appear as red (alkaline phosphatase reaction). Blue hematoxylin counterstain shows PCNA-negative nuclei (original magnification, 125 ×). E, Double immunolabeling for PCNA and vWF. Note abundant microvascularity within thickened intima. Arrowhead indicates a proliferating endothelial cell (original magnification, 125 ×). F, Double immunolabeling for PCNA and CD68/CD45RB. Arrowhead indicates a proliferating inflammatory cell on the left as indicated by a black nucleus and a red cell-type-specific protein cytoplasm (original magnification, 125 ×). Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Morphologic assessment of human vein graft stenoses by hematoxylin and eosin (H&E), Masson's trichrome stain; single-label immunocytochemistry with anti-PCNA, double immunolabeling for PCNA and different cell-specific markers. A, Hypercellularity noted on H&E stain with severe luminal narrowing (original magnification, 3.125 ×). B, Abundant extracellular matrix, high collagen content (blue-green), as seen with Masson's trichrome stain (original magnification, 12.5 ×). C, Immunocytochemical labeling for PCNA. PCNA-positive nuclei appear as dark red and are present in cells adjacent to the residual lumen in this specimen (original magnification, 31 ×). D, Double immunolabeling for PCNA and α-smooth muscle actin. Here PCNA-positive nuclei appear as dark black (peroxidase reaction, shown with arrowhead) and α-smooth muscle actin–positive cytoplasms appear as red (alkaline phosphatase reaction). Blue hematoxylin counterstain shows PCNA-negative nuclei (original magnification, 125 ×). E, Double immunolabeling for PCNA and vWF. Note abundant microvascularity within thickened intima. Arrowhead indicates a proliferating endothelial cell (original magnification, 125 ×). F, Double immunolabeling for PCNA and CD68/CD45RB. Arrowhead indicates a proliferating inflammatory cell on the left as indicated by a black nucleus and a red cell-type-specific protein cytoplasm (original magnification, 125 ×). Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Morphologic assessment of human vein graft stenoses by hematoxylin and eosin (H&E), Masson's trichrome stain; single-label immunocytochemistry with anti-PCNA, double immunolabeling for PCNA and different cell-specific markers. A, Hypercellularity noted on H&E stain with severe luminal narrowing (original magnification, 3.125 ×). B, Abundant extracellular matrix, high collagen content (blue-green), as seen with Masson's trichrome stain (original magnification, 12.5 ×). C, Immunocytochemical labeling for PCNA. PCNA-positive nuclei appear as dark red and are present in cells adjacent to the residual lumen in this specimen (original magnification, 31 ×). D, Double immunolabeling for PCNA and α-smooth muscle actin. Here PCNA-positive nuclei appear as dark black (peroxidase reaction, shown with arrowhead) and α-smooth muscle actin–positive cytoplasms appear as red (alkaline phosphatase reaction). Blue hematoxylin counterstain shows PCNA-negative nuclei (original magnification, 125 ×). E, Double immunolabeling for PCNA and vWF. Note abundant microvascularity within thickened intima. Arrowhead indicates a proliferating endothelial cell (original magnification, 125 ×). F, Double immunolabeling for PCNA and CD68/CD45RB. Arrowhead indicates a proliferating inflammatory cell on the left as indicated by a black nucleus and a red cell-type-specific protein cytoplasm (original magnification, 125 ×). Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Morphologic assessment of human vein graft stenoses by hematoxylin and eosin (H&E), Masson's trichrome stain; single-label immunocytochemistry with anti-PCNA, double immunolabeling for PCNA and different cell-specific markers. A, Hypercellularity noted on H&E stain with severe luminal narrowing (original magnification, 3.125 ×). B, Abundant extracellular matrix, high collagen content (blue-green), as seen with Masson's trichrome stain (original magnification, 12.5 ×). C, Immunocytochemical labeling for PCNA. PCNA-positive nuclei appear as dark red and are present in cells adjacent to the residual lumen in this specimen (original magnification, 31 ×). D, Double immunolabeling for PCNA and α-smooth muscle actin. Here PCNA-positive nuclei appear as dark black (peroxidase reaction, shown with arrowhead) and α-smooth muscle actin–positive cytoplasms appear as red (alkaline phosphatase reaction). Blue hematoxylin counterstain shows PCNA-negative nuclei (original magnification, 125 ×). E, Double immunolabeling for PCNA and vWF. Note abundant microvascularity within thickened intima. Arrowhead indicates a proliferating endothelial cell (original magnification, 125 ×). F, Double immunolabeling for PCNA and CD68/CD45RB. Arrowhead indicates a proliferating inflammatory cell on the left as indicated by a black nucleus and a red cell-type-specific protein cytoplasm (original magnification, 125 ×). Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Morphologic assessment of human vein graft stenoses by hematoxylin and eosin (H&E), Masson's trichrome stain; single-label immunocytochemistry with anti-PCNA, double immunolabeling for PCNA and different cell-specific markers. A, Hypercellularity noted on H&E stain with severe luminal narrowing (original magnification, 3.125 ×). B, Abundant extracellular matrix, high collagen content (blue-green), as seen with Masson's trichrome stain (original magnification, 12.5 ×). C, Immunocytochemical labeling for PCNA. PCNA-positive nuclei appear as dark red and are present in cells adjacent to the residual lumen in this specimen (original magnification, 31 ×). D, Double immunolabeling for PCNA and α-smooth muscle actin. Here PCNA-positive nuclei appear as dark black (peroxidase reaction, shown with arrowhead) and α-smooth muscle actin–positive cytoplasms appear as red (alkaline phosphatase reaction). Blue hematoxylin counterstain shows PCNA-negative nuclei (original magnification, 125 ×). E, Double immunolabeling for PCNA and vWF. Note abundant microvascularity within thickened intima. Arrowhead indicates a proliferating endothelial cell (original magnification, 125 ×). F, Double immunolabeling for PCNA and CD68/CD45RB. Arrowhead indicates a proliferating inflammatory cell on the left as indicated by a black nucleus and a red cell-type-specific protein cytoplasm (original magnification, 125 ×). Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Morphologic assessment of human vein graft stenoses by hematoxylin and eosin (H&E), Masson's trichrome stain; single-label immunocytochemistry with anti-PCNA, double immunolabeling for PCNA and different cell-specific markers. A, Hypercellularity noted on H&E stain with severe luminal narrowing (original magnification, 3.125 ×). B, Abundant extracellular matrix, high collagen content (blue-green), as seen with Masson's trichrome stain (original magnification, 12.5 ×). C, Immunocytochemical labeling for PCNA. PCNA-positive nuclei appear as dark red and are present in cells adjacent to the residual lumen in this specimen (original magnification, 31 ×). D, Double immunolabeling for PCNA and α-smooth muscle actin. Here PCNA-positive nuclei appear as dark black (peroxidase reaction, shown with arrowhead) and α-smooth muscle actin–positive cytoplasms appear as red (alkaline phosphatase reaction). Blue hematoxylin counterstain shows PCNA-negative nuclei (original magnification, 125 ×). E, Double immunolabeling for PCNA and vWF. Note abundant microvascularity within thickened intima. Arrowhead indicates a proliferating endothelial cell (original magnification, 125 ×). F, Double immunolabeling for PCNA and CD68/CD45RB. Arrowhead indicates a proliferating inflammatory cell on the left as indicated by a black nucleus and a red cell-type-specific protein cytoplasm (original magnification, 125 ×). Journal of Vascular Surgery 1997 25, 64-73DOI: (10.1016/S0741-5214(97)70322-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions