Luke W. Hosack, MD, MS, Derek J. Overstreet, PhD, Evan S. Lederman, MD

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Presentation transcript:

In vitro susceptibility of Propionibacterium acnes to simulated intrawound vancomycin concentrations Luke W. Hosack, MD, MS, Derek J. Overstreet, PhD, Evan S. Lederman, MD JSES Open Access Volume 1, Issue 3, Pages 125-128 (October 2017) DOI: 10.1016/j.jses.2017.08.001 Copyright © 2017 The Authors Terms and Conditions

Figure 1 First-order treatment of previous intrawound vancomycin concentrations achieved by Johnson et al10 yielded a linear equation with R2 = 1 (A). With this equation, vancomycin concentrations at any point in time can be calculated and used in vitro to simulate in vivo conditions (B). JSES Open Access 2017 1, 125-128DOI: (10.1016/j.jses.2017.08.001) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Representative blood agar plate showing positive controls (top half) and experimental samples (bottom half). Note the halo surrounding the positive controls, denoting beta-hemolytic activity. JSES Open Access 2017 1, 125-128DOI: (10.1016/j.jses.2017.08.001) Copyright © 2017 The Authors Terms and Conditions

Figure 3 Propionibacterium acnes time-kill curve using simulated in vivo intrawound vancomycin concentrations. Note that the experimental samples from 0 to 9 hours all grew to confluence, as did all the positive controls. Between 9 and 12 hours, colony-forming unit (CFU) counts dropped into a range that allowed quantification. Although bactericidal activity could not be confirmed on the basis of initial inoculum counts, it could be confirmed from time points 9-12 to 24-48 hours, based on a 3 log10 drop in colony-forming units per milliliter. P. acnes was completely eradicated somewhere between 2 and 3 days of treatment. Error bars = ± standard deviation. JSES Open Access 2017 1, 125-128DOI: (10.1016/j.jses.2017.08.001) Copyright © 2017 The Authors Terms and Conditions