Supplementary Figure 2. AKT1 AKT1 AKT2 pAKT ERK pERK A1847 A2780 CaOV3

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Supplementary Figure 2. AKT1 AKT1 AKT2 pAKT ERK pERK A1847 A2780 CaOV3 ES-2 IGROV1 OVCAR3 OVCAR5 OVCAR10 OV-90 SKOV3 PEO-1 TOV-21G TOV-112D 2008 AKT1 (1 minute exposure) AKT1 (10 min exposure) AKT2 pAKT ERK pERK Supplementary Figure 2. Western blot analysis of ovarian cancer cell lines. Cell lines indicated above each lane were analyzed for expression of AKT1, AKT2, pAKT, ERK and pERK expression. Antibodies against AKT, AKT2, and pAKT were used at a 1:500 dilution in overnight incubations. Antibodies against ERK and pERK were used at a 1:1000 dilution. To show the full range of AKT expression, a 1 and 10 minute exposure of the same film is included. A representative result of at least three repeat analyses is shown.